Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

CORC > 中国农业科学院 > 作物科学研究所

	Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)
	Wang Xinmin 1; Ren Xian 2; Yin Guixiang 1; Wang Ke 1; Li Jiarui 3; Du Lipu 1; Xu Huijun 1; Ye Xingguo 1
刊名	Journal of Integrative Agriculture
	2014
卷号	13 期号:4 页码:722-732
关键词	wheat immature embryos environmental temperature preservation plant regeneration
ISSN号	2095-3119
英文摘要	The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4℃ daily cooler for a short period time for its use in plant tissue culture or transformation experiments. This study aimed to investigate the effects of environmental temperature, cryopreservation storage temperature, and heat shock culture (HSC) temperature on the regeneration frequency of wheat IEs. Results indicated that environmental temperature significantly affected the induction of embryonic calli. The optimum total accumulated temperature (TAT) during the time of anthesis and sampling for regeneration of these tissues was around 280℃ for spring wheat type cv. CB037 and approximately 300℃ for winter wheat type cv. Kenong 199. Regeneration ability obviously declined when the highest environmental temperature was over 35℃ for 1 d or a high temperature between 30 and 33℃ lasted for 5 d during anthesis and sampling. This finding was verified by culturing the freshly isolated IEs under different temperatures from 29 to 37℃ in different controlled growth incubators for 5 d; the IEs almost completely lost regeneration ability when the temperature rose to 37℃. Cryopreservation of -20℃ caused the wheat samples lost ability of producing callus or embryonic callus in a few days, and cryopreservation of -10℃ more than 10 d made the regeneration potential of the tissues dramatically declined. Comparatively, the temperature that best maintained high regeneration ability was -5℃, at which the materials can be maintained for around 1 mon. In addition, the preservation of the immature samples at -5 or -10℃ inhibited the direct germination of the IEs, avoiding the embryo axis removing process. Our results are useful for ensuring that field collection and cryopreservation of the wheat IEs are done correctly to enable tissue culture and genetic transformation.
学科主题	物理学 ; 农作物
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106084]
专题	作物科学研究所
作者单位	1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Key Facility of Crop Gene Resources and Genetic Improvement 2.Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081 3.College of Life and Engineering Sciences, Beifang University of Nationalities, Yinchuan, 750021 4.Department of Plant Pathology, Kansas State University, USA, Manhattan, 66506
推荐引用方式 GB/T 7714	Wang Xinmin,Ren Xian,Yin Guixiang,et al. Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture,2014,13(4):722-732.
APA	Wang Xinmin.,Ren Xian.,Yin Guixiang.,Wang Ke.,Li Jiarui.,...&Ye Xingguo.(2014).Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.).Journal of Integrative Agriculture,13(4),722-732.
MLA	Wang Xinmin,et al."Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)".Journal of Integrative Agriculture 13.4(2014):722-732.