Comparative Analysis of Gene Expression Proifling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max

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	Comparative Analysis of Gene Expression Proifling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max
	Li Bin 1; Sun Junming 1; Wang Lan 1; Zhao Rongjuan 1; Wang Lianzheng 1
刊名	Journal of Integrative Agriculture
	2014
卷号	13 期号:12 页码:2594-2607
关键词	soybean soybean cyst nematode Affymetrix Soybean Genome GeneChip gene ontology
ISSN号	2095-3119
英文摘要	Soybean cyst nematode (SCN) is one of the most devastating pathogen for soybean. Therefore, identiifcation of resistant germplasm resources and resistant genes is needed to improve SCN resistance for soybean. Soybean varieties Huipizhiheidou and Wuzhaiheidou were distributed in China and exhibited broad spectrums of resistance to various SCN races. In this study, these two resistant varieties, combined with standard susceptible varieties (Lee and Essex), were utilized to identify the differentially expressed transcripts after infection with SCN race 4 between resistant and susceptible reactions by using the Affymetrix Soybean Genome GeneChip. Comparative analyses indicated that 21 common genes changed signiifcantly in the resistant group, of which 16 increased and 5 decreased. However, 12 common genes changed signiifcantly in the susceptible group, of which 9 increased and 3 decreased. Additionally, 27 genes were found in common between resistant and susceptible reactions. The 21 signiifcantly changed genes in resistant reaction were associated with disease and defense, cell structure, transcription, metabolism, and signal transduction. The fold induction of 4 from the 21 genes was conifrmed by quantitative RT-PCR (qRT-PCR) analysis. Moreover, the gene ontology (GO) enrichment analyses demonstrated the serine family amino acid metabolic process and arginine metabolic process may play important roles in SCN resistance. This study provided a new insight on the genetic basis of soybean resistance to SCN race 4, and the identiifed resistant or resistant-related genes are potentially useful for SCN-resistance breeding in soybean.
学科主题	植物保护
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106073]
专题	作物科学研究所
作者单位	1.MOA/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI 2.Key Laboratory of Soybean Biology Beijing, Beijing, 100081
推荐引用方式 GB/T 7714	Li Bin,Sun Junming,Wang Lan,et al. Comparative Analysis of Gene Expression Proifling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max[J]. Journal of Integrative Agriculture,2014,13(12):2594-2607.
APA	Li Bin,Sun Junming,Wang Lan,Zhao Rongjuan,&Wang Lianzheng.(2014).Comparative Analysis of Gene Expression Proifling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max.Journal of Integrative Agriculture,13(12),2594-2607.
MLA	Li Bin,et al."Comparative Analysis of Gene Expression Proifling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max".Journal of Integrative Agriculture 13.12(2014):2594-2607.