Transgenic maize plants expressing a fungal phytase gene

doi:10.1007/s11248-007-9138-3

CORC > 中国农业科学院 > 作物科学研究所

	Transgenic maize plants expressing a fungal phytase gene
	Chen, Rumei 1; Xue, Guangxing 2; Chen, Ping 1; Yao, Bin 3; Yang, Wenzhu 1; Ma, Qianli 1; Fan, Yunliu 1; Zhao, Zuoyu 4; Tarczynski, Mitchell C.4; Shi, Jinrui 4
刊名	TRANSGENIC RESEARCH
	2008
卷号	17 期号:4 页码:633-643
关键词	maize phytate fungal phytase phyA2 transgenic plant
ISSN号	0962-8819
DOI	10.1007/s11248-007-9138-3
通讯作者	Chen, Rumei
英文摘要	Maize seeds are the major ingredient of commercial pig and poultry feed. Phosphorus in maize seeds exists predominately in the form of phytate. Phytate phosphorus is not available to monogastric animals and phosphate supplementation is required for optimal animal growth. Undigested phytate in animal manure is considered a major source of phosphorus pollution to the environment from agricultural production. Microbial phytase produced by fermentation as a feed additive is widely used to manage the nutritional and environmental problems caused by phytate, but the approach is associated with production costs for the enzyme and requirement of special cares in feed processing and diet formulation. An alternative approach would be to produce plant seeds that contain high phytase activities. We have over-expressed Aspergillus niger phyA2 gene in maize seeds using a construct driven by the maize embryo-specific globulin-1 promoter. Low-copy-number transgenic lines with simple integration patterns were identified. Western-blot analysis showed that the maize-expressed phytase protein was smaller than that expressed in yeast, apparently due to different glycosylation. Phytase activity in transgenic maize seeds reached approximately 2,200 units per kg seed, about a 50-fold increase compared to non-transgenic maize seeds. The phytase expression was stable across four generations. The transgenic seeds germinated normally. Our results show that the phytase expression lines can be used for development of new maize hybrids to improve phosphorus availability and reduce the impact of animal production on the environment.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; BIOCHEMICAL RESEARCH METHODS ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000257384900013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5790]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Crop Sci Inst, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, Feed Res Inst, Beijing 100081, Peoples R China 4.A DuPont Co, Pioneer Hi Bred Int Inc, Johnston, IA 50131 USA
推荐引用方式 GB/T 7714	Chen, Rumei,Xue, Guangxing,Chen, Ping,et al. Transgenic maize plants expressing a fungal phytase gene[J]. TRANSGENIC RESEARCH,2008,17(4):633-643.
APA	Chen, Rumei.,Xue, Guangxing.,Chen, Ping.,Yao, Bin.,Yang, Wenzhu.,...&Shi, Jinrui.(2008).Transgenic maize plants expressing a fungal phytase gene.TRANSGENIC RESEARCH,17(4),633-643.
MLA	Chen, Rumei,et al."Transgenic maize plants expressing a fungal phytase gene".TRANSGENIC RESEARCH 17.4(2008):633-643.