Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.)

doi:10.1007/s11103-005-8519-3

CORC > 中国农业科学院 > 作物科学研究所

	Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.)
	Li, ZK; Fu, BY; Gao, YM; Xu, JL; Ali, J; Lafitte, HR; Jiang, YZ; Rey, JD; Vijayakumar, CHM; Maghirang, R
刊名	PLANT MOLECULAR BIOLOGY
	2005
卷号	59 期号:1 页码:33-52
关键词	abiotic stress tolerances genetic networks linkage disequilibrium QTLs
ISSN号	0167-4412
DOI	10.1007/s11103-005-8519-3
通讯作者	Li, ZK
英文摘要	Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20 000 introgression lines (ILs) in three elite rice genetic backgrounds for a wide range of complex traits, including resistances/tolerances to many biotic and abiotic stresses, morpho-agronomic traits, physiological traits, etc., by selective introgression. ILs within each genetic background are phenotypically similar to their recurrent parent but each carries one or a few traits introgressed from a known donor. Together, these ILs contain a significant portion of loci affecting the selected complex phenotypes at which allelic diversity exists in the primary gene pool of rice. A forward genetics strategy was proposed and demonstrated with examples on how to use these ILs for large-scale FG research. Complementary to the genome-wide insertional mutants, these ILs opens a new way for highly efficient discovery, candidate gene identification and cloning of important QTLs for specific phenotypes based on convergent evidence from QTL position, expression profiling, functional and molecular diversity analyses of candidate genes, highlights the importance of genetic networks underlying complex phenotypes in rice that may ultimately lead to more complete understanding of the genetic and molecular bases of quantitative trait variation in rice.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000232498000004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5764]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China;(2)Int Rice Res Inst, Manila, Philippines
推荐引用方式 GB/T 7714	Li, ZK,Fu, BY,Gao, YM,et al. Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.)[J]. PLANT MOLECULAR BIOLOGY,2005,59(1):33-52.
APA	Li, ZK.,Fu, BY.,Gao, YM.,Xu, JL.,Ali, J.,...&Zhu, LH.(2005).Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.).PLANT MOLECULAR BIOLOGY,59(1),33-52.
MLA	Li, ZK,et al."Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.)".PLANT MOLECULAR BIOLOGY 59.1(2005):33-52.