QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects

doi:10.1007/s00122-006-0227-0

CORC > 中国农业科学院 > 作物科学研究所

	QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects
	Wan, XY; Wan, JM; Jiang, L; Wang, JK; Zhai, HQ; Weng, JF; Wang, HL; Lei, CL; Wang, JL; Zhang, X
刊名	THEORETICAL AND APPLIED GENETICS
	2006
卷号	112 期号:7 页码:1258-1270
ISSN号	0040-5752
DOI	10.1007/s00122-006-0227-0
通讯作者	Wan, XY
英文摘要	Grain length in rice plays an important role in determining rice appearance, milling, cooking and eating quality. In this study, the genetic basis of grain length was dissected into six main-effect quantitative trait loci (QTLs) and twelve pairs of epistatic QTLs. The stability of these QTLs was evaluated in four environments using an F-7 recombinant inbred line (RIL) population derived from the cross between a Japonica variety, Asominori, and an Indica variety, IR24. Moreover, chromosome segment substitution lines (CSSLs) harboring each of the six main-effect QTLs were used to evaluate gene action of QTLs across eight environments. A major QTL denoted as qGL-3a, was found to express stably not only in the isogenic background of Asominori but also in the recombinant background of Asominori and IR24 under multiple environments. The IR24 allele at qGL-3a has a positive effect on grain length. Based on the test of advanced backcross progenies, qGL-3a was dissected as a single Mendelian factor, i.e., long rice grain was controlled by a recessive gene gl-3. High-resolution genetic and physical maps were further constructed for fine mapping gl-3 by using 11 simple sequence repeat (SSR) markers designed using sequence information from seven BAC/PAC clones and a BC4F2 population consisting of 2,068 individuals. Consequently, the gl-3 gene was narrowed down to a candidate genomic region of 87.5 kb long defined by SSR markers RMw357 and RMw353 on chromosome 3, which provides a basis for map-based cloning of this gene and for marker-aided QTL pyramiding in rice quality breeding.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000236585600007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5740]
专题	作物科学研究所
作者单位	(1)Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China;(2)Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wan, XY,Wan, JM,Jiang, L,et al. QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects[J]. THEORETICAL AND APPLIED GENETICS,2006,112(7):1258-1270.
APA	Wan, XY.,Wan, JM.,Jiang, L.,Wang, JK.,Zhai, HQ.,...&Guo, XP.(2006).QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects.THEORETICAL AND APPLIED GENETICS,112(7),1258-1270.
MLA	Wan, XY,et al."QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects".THEORETICAL AND APPLIED GENETICS 112.7(2006):1258-1270.