Isolation and Characterization of GmSTY1, a Novel Gene Encoding a Dual-Specificity Protein Kinase in Soybean (Glycine max L.)

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	Isolation and Characterization of GmSTY1, a Novel Gene Encoding a Dual-Specificity Protein Kinase in Soybean (Glycine max L.)
	Zhaoshi Xu; Youzhi Ma; Xianguo Cheng; Lixia Cao; Liancheng Li; Meng Chen
刊名	Journal of Integrative Plant Biology
	2006
卷号	48 期号:7 页码:857-866
关键词	abiotic stress dual-specificity Glycine max serine/threonine/tyrosine protein kinase
ISSN号	1672-9072
英文摘要	Phosphorylation of protein kinases has profound effects on their activity and interaction with other proteins.Tyrosine phosphorylation was reported to be involved in various physiological processes in plants; however,no typical receptor tyrosine kinase has been isolated from plants thus far. Dual-specificity kinases are potentially responsible for the phosphorylation of both tyrosine and serine/threonine of target proteins. A cDNA clone encoding a putative dual-specificity protein kinase was isolated by screening the cDNA GAL4 activation domain (AD) fusion library of soybean (Glycine max L.), and its entire length was obtained using 5'-rapid amplification of cDNA ends. The predicted polypeptide of 330 amino acid residues, designated as GmSTY1, contains all 11 conserved subdomains, which share common characteristics with both the serine/threonine and tyrosine protein kinases reported thus far. In addition, three potential N-linked glycosylation sites (NXS/T), as well as phosphorylation motifs (SXXXS/T), were observed, suggesting that GmSTY1 may be post-translationally modified. Furthermore, a potential N-myristoylation motif (MGARCSK) was found,suggesting that the GmSTY1 protein could associate with membranes In vivo. Southern blotting analysis revealed a single-copy of GmSTY1 in the genome. Northern blotting analysis showed that this gene was upregulated by drought and salt treatment in a time-dependent manner; however, exogenous abscisic acid (ABA) could not significantly affect the mRNA accumulation of GmSTY1. Interestingly, the transcript of this gene was remarkably downregulated by cold treatment during the early stages of the response, but upregulated later. These results indicate that the protein kinase was possibly regulated by abiotlc stresses in an ABA-independent pathway.
学科主题	植物学
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5728]
专题	作物科学研究所
作者单位	Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	Zhaoshi Xu,Youzhi Ma,Xianguo Cheng,et al. Isolation and Characterization of GmSTY1, a Novel Gene Encoding a Dual-Specificity Protein Kinase in Soybean (Glycine max L.)[J]. Journal of Integrative Plant Biology,2006,48(7):857-866.
APA	Zhaoshi Xu,Youzhi Ma,Xianguo Cheng,Lixia Cao,Liancheng Li,&Meng Chen.(2006).Isolation and Characterization of GmSTY1, a Novel Gene Encoding a Dual-Specificity Protein Kinase in Soybean (Glycine max L.).Journal of Integrative Plant Biology,48(7),857-866.
MLA	Zhaoshi Xu,et al."Isolation and Characterization of GmSTY1, a Novel Gene Encoding a Dual-Specificity Protein Kinase in Soybean (Glycine max L.)".Journal of Integrative Plant Biology 48.7(2006):857-866.