Variation in two PPO genes associated with polyphenol oxidase activity in seeds of common wheat

doi:10.1007/s10681-006-9285-2

CORC > 中国农业科学院 > 作物科学研究所

	Variation in two PPO genes associated with polyphenol oxidase activity in seeds of common wheat
	Chang, Cheng; Zhang, Hai-Ping; Xu, Jie; You, Ming-Shan; Li, Bao-Yun; Liu, Guang-Tian
刊名	EUPHYTICA
	2007
卷号	154 期号:1-2 页码:181-193
关键词	common wheat PPO variation chromosome location expression
ISSN号	0014-2336
DOI	10.1007/s10681-006-9285-2
通讯作者	Chang, Cheng
英文摘要	Polyphenol oxidase (PPO) is often regarded as a major factor resulting in time-dependent darkening and discoloration of Asian noodles and other wheat end products. To understand the relationship between variation of PPO genes and PPO activity of seed, the three PPO genes, which express in immature wheat grain, were investigated in 216 common wheat cultivars. The results indicated that only TaPPO-A1 and TaPPO-D1 showed high polymorphisms related to PPO activity. Two alleles in both TaPPO-A1 (TaPPO-A1a and TaPPO-A1b) and TaPPO-D1 (TaPPO-D1a and TaPPO-D1b) were detected using denaturing polyacrylamide gel electrophoresis. Wheat cultivars with TaPPO-A1b usually showed higher PPO activity than those with TaPPO-A1a. The TaPPO-D1a allele was often found in lower-PPO-activity cultivars, compared with TaPPO-D1b. The sequencing results of DNA fragments confirmed that two introns existed in TaPPO-D1 like TaPPO-A1. Some variation of introns was detected in the two alleles of TaPPO-D1. During seed development, the high-PPO-activity cultivar, Yangmai 158 (TaPPO-A1b/TaPPO-D1b) showed higher transcription of the two PPO genes, in comparison to low-activity cultivar, Yongchuanbaimai (TaPPO-A1a/TaPPO-D1a). These results suggested that variation in introns may influence the transcription of TaPPO-A1 and TaPPO-D1 in immature seeds of wheat.
学科主题	Agronomy ; Plant Sciences ; Horticulture ; AGRONOMY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000244191900018
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5676]
专题	作物科学研究所
作者单位	(1)China Agr Univ, Coll Agron & Biotechnol, Beijing 100094, Peoples R China;(2)Minist Agr, Key Lab Crop Genet Improvement, Key Lab Crop Genom & Genet Improvement, Beijing, Peoples R China;(3)Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, NFCRI, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Chang, Cheng,Zhang, Hai-Ping,Xu, Jie,et al. Variation in two PPO genes associated with polyphenol oxidase activity in seeds of common wheat[J]. EUPHYTICA,2007,154(1-2):181-193.
APA	Chang, Cheng,Zhang, Hai-Ping,Xu, Jie,You, Ming-Shan,Li, Bao-Yun,&Liu, Guang-Tian.(2007).Variation in two PPO genes associated with polyphenol oxidase activity in seeds of common wheat.EUPHYTICA,154(1-2),181-193.
MLA	Chang, Cheng,et al."Variation in two PPO genes associated with polyphenol oxidase activity in seeds of common wheat".EUPHYTICA 154.1-2(2007):181-193.