Interaction study of MADS-domain proteins in tomato

doi:10.1093/jxb/ern094

CORC > 中国农业科学院 > 作物科学研究所

	Interaction study of MADS-domain proteins in tomato
	Leseberg, Charles H.1,2,3; Eissler, Christie L.1; Wang, Xiang 2,3; Johns, Mitrick A.1; Duvall, Melvin R.1; Mao, Long 1,2,3
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2008
卷号	59 期号:8 页码:2253-2265
关键词	flower development higher order complexes MADS-domain proteins protein-protein interaction tomato yeast two-hybrid
ISSN号	0022-0957
DOI	10.1093/jxb/ern094
通讯作者	Leseberg, Charles H.
英文摘要	MADS-domain proteins are important transcription factors involved in many biological processes of plants. Interactions between MADS-domain proteins are essential for their functions. In tomato (Solanum lycopersicum), the number of MIKCc-type MADS-domain proteins identified has totalled 36, but a large-scale interaction assay is lacking. In this study, 22 tomato MADS-domain proteins were selected from six functionally important subfamilies of the MADS-box gene family, to create the first large-scale tomato protein interaction network. Compared with Arabidopsis and petunia (Petunia hybrida), protein interaction patterns in tomato displayed both conservation and divergence. The majority of proteins that can be identified as putative orthologues exhibited conserved interaction patterns, and modifications were mostly found in genes underlining traits unique to tomato. JOINTLESS and RIN, characterized for their roles in abscission zone development and fruit ripening, respectively, showed enlarged interaction networks in comparison with their Arabidopsis and petunia counterparts. Novel interactions were also found for members of the expanded subfamilies, such as those represented by AP1/FUL and AP3/PI MADS-domain proteins. In search for higher order complexes, TM5 was found to be the preferred bridge among the five SEP-like proteins. Additionally, 16 proteins with the MADS-domain removed were used to assess the role of the MADS-domain in protein-protein interactions. The current work provides important knowledge for further functional and evolutionary study of the MADS-box genes in tomato.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000256519100024
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5609]
专题	作物科学研究所
作者单位	1.No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Leseberg, Charles H.,Eissler, Christie L.,Wang, Xiang,et al. Interaction study of MADS-domain proteins in tomato[J]. JOURNAL OF EXPERIMENTAL BOTANY,2008,59(8):2253-2265.
APA	Leseberg, Charles H.,Eissler, Christie L.,Wang, Xiang,Johns, Mitrick A.,Duvall, Melvin R.,&Mao, Long.(2008).Interaction study of MADS-domain proteins in tomato.JOURNAL OF EXPERIMENTAL BOTANY,59(8),2253-2265.
MLA	Leseberg, Charles H.,et al."Interaction study of MADS-domain proteins in tomato".JOURNAL OF EXPERIMENTAL BOTANY 59.8(2008):2253-2265.