Proteomics analysis of major royal jelly protein changes under different storage conditions

doi:10.1021/pr8002276

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	Proteomics analysis of major royal jelly protein changes under different storage conditions
	Li, Jian-ke 2; Feng, Mao 2; Zhang, Lan 2; Zhang, Zhao-hui 1; Pan, Ying-hong 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2008
卷号	7 期号:8 页码:3339-3353
关键词	major royal jelly proteins (MRJPs) storage temperature 2-DE MALDI-TOF/MS Nano LC MS/MS
ISSN号	1535-3893
DOI	10.1021/pr8002276
通讯作者	Li, Jian-ke
英文摘要	Protein changes in fresh royal jelly (RJ) were compared when stored at -20, 4 degrees C, and room temperature (RT) for 12 months. Protein was partially identified using combinations of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF/MS), gel filtration chromatography, nanoLC MS/MS, and a protein engine identification tool applied to the honeybee genome. Significantly more protein spots were found in fresh (85 spots) and -20 degrees C (81 spots) stored RJ than in samples stored at 4 degrees C (73 spots) and at RT (70 spots) for 1 year. Most identified spots, 56, 57, 51, 46, corresponding to RJ sample of the fresh, -20 degrees C, 4 degrees C, and RT, were assigned to major royal jelly proteins (MRJPs). Marked differences were found in the heterogeneity of the MRJPs, in particular, MRJP3. The quantity of MRJP1 decreased significantly following the temperature trend in all images, but MRJP 2 and -3 did not increase or decrease following the temperature trend, thus, suggesting that MRJP 1-3 are sensitive to temperature. However, MRJP4, 5, glucose oxidase (GOD), peroxiredoxin (PRDX), and glutathione S-transferase (GST) S1 were clearly absent in all images in samples held at RT for 1 year. This indicates that they are the proteins most sensitive to storage temperature and protein markers for freshness of RJ. Combining chromatography and nanoLC MS/MS results, we tentatively conclude that MRJP5 is a reliable freshness marker and that the best way to maintain quality of RJ is under freezing conditions.
学科主题	Biochemical Research Methods ; BIOCHEMICAL RESEARCH METHODS
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000258200400025
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5590]
专题	作物科学研究所
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Jian-ke,Feng, Mao,Zhang, Lan,et al. Proteomics analysis of major royal jelly protein changes under different storage conditions[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(8):3339-3353.
APA	Li, Jian-ke,Feng, Mao,Zhang, Lan,Zhang, Zhao-hui,&Pan, Ying-hong.(2008).Proteomics analysis of major royal jelly protein changes under different storage conditions.JOURNAL OF PROTEOME RESEARCH,7(8),3339-3353.
MLA	Li, Jian-ke,et al."Proteomics analysis of major royal jelly protein changes under different storage conditions".JOURNAL OF PROTEOME RESEARCH 7.8(2008):3339-3353.