A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat

doi:10.1007/s00299-008-0623-9

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	A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat
	Gao, Shi-Qing; Chen, Ming; Xia, Lian-Qin; Xiu, Hui-Jun; Xu, Zhao-Shi; Li, Lian-Cheng; Zhao, Chang-Ping; Cheng, Xian-Guo; Ma, You-Zhi
刊名	PLANT CELL REPORTS
	2009
卷号	28 期号:2 页码:301-311
关键词	Wheat DREB gene Abiotic stress tolerance
ISSN号	0721-7714
DOI	10.1007/s00299-008-0623-9
通讯作者	Gao, Shi-Qing
英文摘要	A cotton (G. hirsutum L.) dehydration responsive element binding protein gene, GhDREB, which encodes a 153 amino acid protein containing a conserved AP2/EREBP domain, was isolated from the cDNA library of cotton cv. Simian 3 by a yeast one-hybrid system. RNA blot analysis showed that the GhDREB gene was induced in cotton seedlings by drought, high salt and cold stresses. An electrophoretic mobility shift assay (EMSA) indicated that the GhDREB protein bound specifically to the DRE core element (A/GCCGAC) in vitro. Two expression vectors containing the GhDREB gene with either of the Ubiqutin or rd29A promoters were constructed and transferred into wheat (Triticum aestivum L.) by bombardment. Fifty-eight Ubi::GhDREB and 17 rd29A::GhDREB T-0 plants of Yangmai (36 plants) and Lumai (39 plants) were identified by PCR analysis, respectively. Southern blot and RT-PCR analyses showed that two or three copies of the GhDREB were integrated into the Yangmai 10 genome and were expressed at the transcriptional level, and three or four copies were integrated into the Lumai 23 genome. Functional analysis indicated that the transgenic plants had improved tolerance to drought, high salt, and freezing stresses through accumulating higher levels of soluble sugar and chlorophyll in leaves after stress treatments. No phenotype differences were observed between transgenic plants and their non-transgenic controls. These results indicated that GhDREB might be useful in improving wheat stress tolerance through genetic engineering.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000262830500014
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5543]
专题	作物科学研究所
作者单位	Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China
推荐引用方式 GB/T 7714	Gao, Shi-Qing,Chen, Ming,Xia, Lian-Qin,et al. A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat[J]. PLANT CELL REPORTS,2009,28(2):301-311.
APA	Gao, Shi-Qing.,Chen, Ming.,Xia, Lian-Qin.,Xiu, Hui-Jun.,Xu, Zhao-Shi.,...&Ma, You-Zhi.(2009).A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat.PLANT CELL REPORTS,28(2),301-311.
MLA	Gao, Shi-Qing,et al."A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat".PLANT CELL REPORTS 28.2(2009):301-311.