Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz

doi:10.1016/j.scienta.2012.02.013

CORC > 中国农业科学院 > 作物科学研究所

	Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz
	Yi, Shanjun 1; Qian, Yongqiang 1; Han, Lei 1; Sun, Zhenyuan 1; Fan, Chengming 2; Liu, Junxiang 1; Ju, Guansheng 1
刊名	SCIENTIA HORTICULTURAE
	2012
卷号	138 页码:128-133
关键词	Quantitative real-time PCR Reference gene geNorm NormFinder Rhododendron micranthum Turcz
ISSN号	0304-4238
DOI	10.1016/j.scienta.2012.02.013
通讯作者	Yi, Shanjun
英文摘要	Selection of reliable reference genes is a prerequisite for accurate normalization of gene expression in quantitative real-time polymerase chain reaction (qRT-PCR). The present study is the first report of a systematic evaluation of potential reference genes in Rhododendron micranthum Turcz. The expression stabilities of 6 candidate reference genes (185, ACT, CYP, UBQ TUA, and EF1 alpha) in different tissues and the same tissue at different developmental stages were assessed in Rh. micranthum Turcz, using two analysis software packages, geNorm and NormFinder. The results indicated that EF1 alpha and UBQ were the most stable reference genes for different tissues (leaf, root, stem and flower) at the same developmental stage, whereas 18S should be avoided. During leaf development. EF1 alpha and 185 can be used as the most reliable reference genes, but CYP was the most variable one. During flower development, the most stable genes were CYP and EF1 alpha, while 18S was the least stable one. In order to demonstrate the importance of using suitable reference genes for obtaining an accurate and reliable results in gene expression studies, the expression pattern of an independent target gene, RhMDH gene was analyzed in parallel, the RhMDH showed an expression difference of 58-fold in stem when normalized with the most stable gene (EF1 alpha)or the least stable gene (185) act as reference, respectively. Our results support that the selection of suitable reference genes is necessary to achieve accurate results in qRT-PCR. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Horticulture ; HORTICULTURE
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000303623800017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5171]
专题	作物科学研究所
作者单位	1.Chinese Acad Forestry, State Key Lab Forest Genet & Tree Breeding, Beijing 100091, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Yi, Shanjun,Qian, Yongqiang,Han, Lei,et al. Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz[J]. SCIENTIA HORTICULTURAE,2012,138:128-133.
APA	Yi, Shanjun.,Qian, Yongqiang.,Han, Lei.,Sun, Zhenyuan.,Fan, Chengming.,...&Ju, Guansheng.(2012).Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz.SCIENTIA HORTICULTURAE,138,128-133.
MLA	Yi, Shanjun,et al."Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz".SCIENTIA HORTICULTURAE 138(2012):128-133.