Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots

doi:10.4238/2015.June.29.19

CORC > 中国农业科学院 > 作物科学研究所

	Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots
	Li, W. C.1; Ji, S. D.1; Wang, X. C.1; Li, Z. K.1; Zhang, H. C.1; Tian, C. Z.1; Liu, Y. L.1; Duan, C. X.2
刊名	GENETICS AND MOLECULAR RESEARCH
	2015
卷号	14 期号:2 页码:7258-7266
关键词	Ion beam Transformation Rice Proteinase
ISSN号	1676-5680
DOI	10.4238/2015.June.29.19
通讯作者	Li, W. C.
英文摘要	Corn DNA was introduced into dry seeds of rice (cv. 'YuJing-6') by ion beam irradiation. Proteinase activities in rice seedling roots were subsequently analyzed by renaturation electrophoresis at pH 4.5, 7.0, and 8.5. Proteinase activity was more pronounced on gels at higher pH. Irradiation of rice seedling roots caused the loss of some proteinase bands at all pH conditions although a novel 50-kDa band was found at both pH 7.0 and 8.5. No new proteinase activity was detected at pH 4.5. However, novel bands and bands showing stronger activity were observed at pH 7.0 and 8.5. The data indicate that the expression of proteinases in rice seedling roots was altered following low energy ion beam mediated transformation with corn DNA.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	FUNPEC-EDITORA
WOS记录号	WOS:000357189800166
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4783]
专题	作物科学研究所
作者单位	1.Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, W. C.,Ji, S. D.,Wang, X. C.,et al. Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots[J]. GENETICS AND MOLECULAR RESEARCH,2015,14(2):7258-7266.
APA	Li, W. C..,Ji, S. D..,Wang, X. C..,Li, Z. K..,Zhang, H. C..,...&Duan, C. X..(2015).Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots.GENETICS AND MOLECULAR RESEARCH,14(2),7258-7266.
MLA	Li, W. C.,et al."Ion beam transformation with corn DNA alters proteinase expression in rice seedling roots".GENETICS AND MOLECULAR RESEARCH 14.2(2015):7258-7266.