NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis

doi:10.1093/nar/gku1325

CORC > 中国农业科学院 > 作物科学研究所

	NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis
	Gao, Wei; Liu, Wenwen; Zhao, Meng; Li, Wen-Xue
刊名	NUCLEIC ACIDS RESEARCH
	2015
卷号	43 期号:1 页码:607-617
ISSN号	0305-1048
DOI	10.1093/nar/gku1325
通讯作者	Gao, Wei
英文摘要	NFYA5 is an important drought-stress inducible transcription factor gene that is targeted by miR169 in Arabidopsis. We show here that the cis-natural antisense transcript gene of NFYA5, NFYA5 Enhanc-ing RING FINGER (NERF), can produce siRNAs from their overlapping region (OR) and affect NFYA5 transcripts by functioning together with miR169. The NERF protein functions as an E3 ligase for ubiquitination. Overexpression of NERF or OR cDNA leads to siRNANERF accumulation, miR169 repression, and NFYA5 transcript enhancement; knockdown of NERF transcripts by an artificial miRNA enhances miR169 abundance and reduces NFYA5 transcripts. Overexpression of NFYA5 does not affect the NERF mRNA level. Deep sequencing of the small RNA library from 35S::OR plants identifies 960 sequences representing 323 unique siRNAs that originate from OR; the sequences of some siRNANERF are similar/complementary to those of miR169. Overexpression of the 195-to 280-bp OR cDNA-containing siRNAs similar/complementary to miR169 also leads to the accumulation of NFYA5 transcripts. Analysis of NERF knock-down plants and NERF overexpression lines showed that, like NFYA5, NERF is important for controlling stomatal aperture and drought resistance. This regulatory model might apply to other natural antisense transcripts with positively correlated expression patterns.
学科主题	Biochemistry & Molecular Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000350207100056
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4774]
专题	作物科学研究所
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Gao, Wei,Liu, Wenwen,Zhao, Meng,et al. NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis[J]. NUCLEIC ACIDS RESEARCH,2015,43(1):607-617.
APA	Gao, Wei,Liu, Wenwen,Zhao, Meng,&Li, Wen-Xue.(2015).NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis.NUCLEIC ACIDS RESEARCH,43(1),607-617.
MLA	Gao, Wei,et al."NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis".NUCLEIC ACIDS RESEARCH 43.1(2015):607-617.