Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death

doi:10.1111/plb.12271

CORC > 中国农业科学院 > 作物科学研究所

	Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death
	Lin, D.1; Jiang, Q.1; Zheng, K.1; Chen, S.1,2; Zhou, H.1,2; Gong, X.1; Xu, J.3; Teng, S.2; Dong, Y.1
刊名	PLANT BIOLOGY
	2015
卷号	17 期号:3 页码:599-607
关键词	Chloroplast development chloroplast ribosomal protein gene mutation map-based cloning rice (Oryza sativa L )
ISSN号	1435-8603
DOI	10.1111/plb.12271
通讯作者	Lin, D.
英文摘要	The plastid ribosome proteins (PRPs) play important roles in plastid protein biosynthesis, chloroplast differentiation and early chloroplast development. However, the specialised functions of individual protein components of the chloroplast ribosome in rice (Oryza sativa) remain unresolved. In this paper, we identified a novel rice PRP mutant named asl2 (Albino seedling lethality 2) exhibiting an albino, seedling death phenotype. In asl2 mutants, the alteration of leaf colour was associated with chlorophyll (Chl) content and abnormal chloroplast development. Through map-based cloning and complementation, the mutated ASL2 gene was isolated and found to encode the chloroplast 50S ribosome protein L21 (RPL21c), a component of the chloroplast ribosome large subunit, which was localised in chloroplasts. ASL2 was expressed at a higher level in the plumule and leaves, implying its tissue-specific expression. Additionally, the expression of ASL2 was regulated by light. The transcript levels of the majority of genes for Chl biosynthesis, photosynthesis and chloroplast development were strongly affected in asl2 mutants. Collectively, the absence of functional ASL2 caused chloroplast developmental defects and seedling death. This report establishes the important role of RPL21c in chloroplast development in rice.
学科主题	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000353057300002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4716]
专题	作物科学研究所
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Lin, D.,Jiang, Q.,Zheng, K.,et al. Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death[J]. PLANT BIOLOGY,2015,17(3):599-607.
APA	Lin, D..,Jiang, Q..,Zheng, K..,Chen, S..,Zhou, H..,...&Dong, Y..(2015).Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death.PLANT BIOLOGY,17(3),599-607.
MLA	Lin, D.,et al."Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death".PLANT BIOLOGY 17.3(2015):599-607.