Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane | |
Fan, Jinxin1,2; Luo, Jianquan1,2; Wan, Yinhua1,2 | |
刊名 | JOURNAL OF MEMBRANE SCIENCE |
2017-09-15 | |
卷号 | 538页码:68-76 |
关键词 | Enzymatic Membrane Reactor Membrane Chromatography Laccase Membrane Adsorber Bisphenol a |
ISSN号 | 0376-7388 |
DOI | 10.1016/j.memsci.2017.05.053 |
文献子类 | Article |
英文摘要 | Enzyme purification and subsequent immobilization usually require multiple steps and consume plenty of chemicals. Traditional dipping incubation operation is lengthy and the interior of porous carrier is not fully exploited due to the diffusion barrier. To solve these problems, one-step enzyme purification and immobilization was developed based on membrane chromatography concept. A membrane adsorber was prepared by polydopamine coating on a PVDF microfiltration membrane (or on a cheap and disposable cellulose filter paper) and subsequent polyethyleneimine grafting, without any solvent usage. Laccase was selectively immobilized on such membrane adsorber (enzyme activity: 39.9 +/- 4.4 U/mL; expressed activity: 19.6 +/- 3.0%) by directly capturing it from a crude fermentation broth without pH adjustment under flow through mode. The immobilized laccase had a high purity of 92.2%, while the membrane permeability decreased due to the adsorption of laccase and impurities ("membrane fouling"). Thanks to the dominant convective transport, the enzyme loading was high and much faster than that by dipping incubation (5 min vs. 80 min). Moreover, enzyme reloading on membrane adsorber was easily realized by reversible desorption-adsorption process. The constructed biocatalytic membrane exhibited a commendable reusability for bisphenol A (BPA) removal only driven by its gravity. |
WOS关键词 | Bisphenol-a Removal ; Laccase Immobilization ; Protein Adsorption ; Performance ; Exchange ; Degradation ; Carbon ; Polydopamine ; Separation ; Supports |
WOS研究方向 | Engineering ; Polymer Science |
语种 | 英语 |
WOS记录号 | WOS:000402509500008 |
资助机构 | National Natural Science Foundation of China(21506229) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/22656] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Fan, Jinxin,Luo, Jianquan,Wan, Yinhua. Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane[J]. JOURNAL OF MEMBRANE SCIENCE,2017,538:68-76. |
APA | Fan, Jinxin,Luo, Jianquan,&Wan, Yinhua.(2017).Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane.JOURNAL OF MEMBRANE SCIENCE,538,68-76. |
MLA | Fan, Jinxin,et al."Membrane chromatography for fast enzyme purification, immobilization and catalysis: A renewable biocatalytic membrane".JOURNAL OF MEMBRANE SCIENCE 538(2017):68-76. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论