Real-time PCR microfluidic devices with concurrent electrochemical detection

	Real-time PCR microfluidic devices with concurrent electrochemical detection
	Fang TH ; Ramalingam N ; Dong XD ; Ngin TS ; Zeng XT ; Kuan ATL ; Huat EYP ; Gong HQ
刊名	biosensors & bioelectronics
	2009
卷号	24 期号:7 页码:2131-2136
关键词	METHYLENE-BLUE DNA AMPLIFICATION ELECTRODES CHIP
ISSN号	0956-5663
通讯作者	gong hq
中文摘要	electrochemistry-based detection methods hold great potential towards development of hand-held nucleic-acid analyses instruments. in this work, we demonstrate the implementation of in situ electrochemical (ec) detection method in a microfluidic flow-through ec-qpcr (ftec-qpcr) device, where both the amplification of the target nucleic-acid sequence and subsequent ec detection of the pcr amplicon are realized simultaneously at selected pcr cycles in the same device. the ftec-qpcr device utilizes methylene blue (mb), an electroactive dna intercalator, for electrochemical signal measurements in the presence of pcr reagent components. our ec detection method is advantageous, when compared to other existing ec methods for pcr amplicon analysis, since ftec-qpcr does not require probe-modified electrodes, or asymmetric pcr, or solid-phase pcr. key technical issues related to surface passivation, electrochemical measurement, pcr inhibition by metal electrode, bubble-free pcr, were investigated. by controlling the concentration of mb and the exposure of pcr mixture to the bare metal electrode, we successfully demonstrated electrochemical measurement of mb in solution-phase, symmetric pcr by amplifying a fragment of lambda phage dna.
收录类别	SCI
语种	英语
WOS记录号	WOS:000264791000052
公开日期	2010-06-18
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/12909]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Fang TH,Ramalingam N,Dong XD,et al. Real-time PCR microfluidic devices with concurrent electrochemical detection[J]. biosensors & bioelectronics,2009,24(7):2131-2136.
APA	Fang TH.,Ramalingam N.,Dong XD.,Ngin TS.,Zeng XT.,...&Gong HQ.(2009).Real-time PCR microfluidic devices with concurrent electrochemical detection.biosensors & bioelectronics,24(7),2131-2136.
MLA	Fang TH,et al."Real-time PCR microfluidic devices with concurrent electrochemical detection".biosensors & bioelectronics 24.7(2009):2131-2136.