Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE)

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	Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE)
	Fang, Bo 1,2; Xu, Huifang 1; Liu, Yi 1; Qi, Fengxia 1; Zhang, Wei 1; Chen, Hui 1; Wang, Cong 1; Wang, Yilin 1,2; Yang, Wenxia 1; Li, Shengying 1
刊名	SCIENTIFIC REPORTS
	2017-03-09
卷号	7
英文摘要	The cytochrome P450 enzyme OleT(JE) from Jeotgalicoccus sp. ATCC 8456 is capable of converting free long-chain fatty acids into alpha-alkenes via one-step oxidative decarboxylation in presence of H2O2 as cofactor or using redox partner systems. This enzyme has attracted much attention due to its intriguing but unclear catalytic mechanism and potential application in biofuel production. Here, we investigated the functionality of a select group of residues (Arg245, Cys365, His85, and Ile170) in the active site of OleTJE through extensive mutagenesis analysis. The key roles of these residues for catalytic activity and reaction type selectivity were identified. In addition, a range of heterologous redox partners were found to be able to efficiently support the decarboxylation activity of OleTJE. The best combination turned out to be SeFdx-6 (ferredoxin) from Synechococcus elongatus PCC 7942 and CgFdR-2 (ferredoxin reductase) from Corynebacterium glutamicum ATCC 13032, which gave the highest myristic acid conversion rate of 94.4%. Moreover, Michaelis-Menton kinetic parameters of OleTJE towards myristic acid were determined.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	CYTOCHROME-P450 ENZYMES ; OXIDATIVE DECARBOXYLATION ; SUBSTRATE RECOGNITION ; BACILLUS-SUBTILIS ; CRYSTAL-STRUCTURE ; HYDROXYLATION ; PEROXYGENASE ; CHLOROPEROXIDASE ; PEROXIDASE ; ALKENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000395976400001
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9344]
专题	青岛生物能源与过程研究所_酶工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fang, Bo,Xu, Huifang,Liu, Yi,et al. Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE)[J]. SCIENTIFIC REPORTS,2017,7.
APA	Fang, Bo.,Xu, Huifang.,Liu, Yi.,Qi, Fengxia.,Zhang, Wei.,...&Li, Shengying.(2017).Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE).SCIENTIFIC REPORTS,7.
MLA	Fang, Bo,et al."Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE)".SCIENTIFIC REPORTS 7(2017).