Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy

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	Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy
	Tao, Yifan 1,2,3; Wang, Yun 2,3; Huang, Shi 2,3; Zhu, Pengfei 2,3; Huang, Wei E.4; Ling, Junqi 1; Xu, Jian 2,3,5
刊名	ANALYTICAL CHEMISTRY
	2017-04-04
卷号	89 期号:7 页码:4108-4115
英文摘要	To combat the spread of antibiotic resistance, methods that quantitatively assess the metabolism-inhibiting effects of drugs in a rapid and culture-independent manner are urgently needed. Here using four oral bacteria as models, we show that heavy water (D2O)-based single-cell Raman micro- spectroscopy (D2O-Raman) can probe bacterial response to different drugs using the Raman shift at the C-D (carbon deuterium vibration) band in 2040 to 2300 cm(-1) as a universal biomarker for metabolic activity at single-bacterial-cell resolution. The "minimum inhibitory concentration based on metabolic activity" (MIC-MA), defined as the minimal dose under which the median Delta CD-ratio at 8 h of drug exposure is <= 0 and the standard deviation (SD) of the Delta C-D ratio among, individual cells is <= 0.005, was proposed to evaluate the metabolism-inhibiting efficacy of drugs. In addition, heterogeneity index of MIC-MA (MIC-MA-HI), defined as SD of C-D ratio, among individual cells, quantitatively assesses the among-cell heterogeneity of metabolic activity after drug regimens. When exposed to 1 X MIC of sodium fluoride (NaF), 1 X MIC of chlorhexidine (CHX), or 60 X MIC of ampicillin, the cariogenic oral pathogen Streptococcus mutans UA159 ceased propagation yet remained metabolically highly active. This underscores the advantage of MIC-MA over the growth-based MIC in being able to detect the "nongrowing but metabolically active" (NGMA) cells that underlie many latent or recurring infections. Moreover, antibiotic susceptible and resistant S. mutans strains can be readily discriminated at as early as 0.5 h. Thus, D2O-Raman can serve as a universal method for rapid and quantitative assessment of antimicrobial effects based on general metabolic activity at single-cell resolution.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	MYCOBACTERIUM-TUBERCULOSIS ; PHENOTYPIC HETEROGENEITY ; STREPTOCOCCUS-MUTANS ; ESCHERICHIA-COLI ; IN-VITRO ; RESISTANCE ; STRESS ; BIOLUMINESCENCE ; SPECTROSCOPY ; INHIBITION
收录类别	SCI
语种	英语
WOS记录号	WOS:000398645300043
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9298]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Affiliated Stomatol Hosp, Operat Dent & Endodont,Guanghua Sch Stomatol, Guangzhou 510055, Guangdong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 4.Univ Oxford, Dept Engn Sci, Parks Rd, Oxford OX1 3PJ, England 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Tao, Yifan,Wang, Yun,Huang, Shi,et al. Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy[J]. ANALYTICAL CHEMISTRY,2017,89(7):4108-4115.
APA	Tao, Yifan.,Wang, Yun.,Huang, Shi.,Zhu, Pengfei.,Huang, Wei E..,...&Xu, Jian.(2017).Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy.ANALYTICAL CHEMISTRY,89(7),4108-4115.
MLA	Tao, Yifan,et al."Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy".ANALYTICAL CHEMISTRY 89.7(2017):4108-4115.