Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase

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	Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase
	Gu, Jing 1; Chen, Yuanyuan 1,2; Guo, Hongsen 1,2; Sun, Manluan 1,3; Yang, Mingkun 4; Wang, Xude 1; Zhang, Xian'en 5; Deng, Jiaoyu 1
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2017-02-01
卷号	49 期号:2 页码:186-192
关键词	pyridoxine 5 '-phosphate oxidase lysine acetylation CobB deacetylation
ISSN号	1672-9145
英文摘要	N epsilon-lysine acetylation is one of the most abundant post-translational modifications in eukaryote and prokaryote. Protein acetylome of Escherichia coli has been screened using mass spectrometry (MS) technology, and many acetylated proteins have been identified, including the pyridoxine 5'-phosphate oxidase (EcPNPOx), but the biological roles played by lysine acetylation in EcPNPOx still remain unknown. In this study, EcPNPOx was firstly overexpressed and purified, and two acetylated lysine residues were identified by the subsequent liquid chromatography-tandem mass spectrometry analysis. Site-directed mutagenesis analysis demonstrated that acetylated lysine residues play important roles in the enzymatic activity and enzymatic properties of the protein. EcPNPOx could be non-enzymatically acetylated by acetyl-phosphate and deacetylated by CobB in vitro. Furthermore, enzymatic activities of acetylated and deacetylated EcPNPOx were compared in vitro, and results showed that acetylation led to a decrease of its enzymatic activity, which could be rescued by CobB deacetylation. Taken together, our data suggest that CobB modulates the enzymatic activity of EcPNPOx in vitro.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	X-RAY-STRUCTURE ; PROTEIN ACETYLATION ; PYRIDOXINE-5'-PHOSPHATE OXIDASE ; 5-PHOSPHATE OXIDASE ; ANGSTROM RESOLUTION ; RABBIT LIVER ; E. COLI ; COBB ; BIOSYNTHESIS ; VITAMIN-B6
收录类别	SCI
语种	英语
WOS记录号	WOS:000397088800010
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/29053]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Huazhong Agr Univ, Wuhan 430070, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China 5.Chinese Acad Sci, Nat Lab Biomacromolecules, Inst Biophys, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Gu, Jing,Chen, Yuanyuan,Guo, Hongsen,et al. Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2017,49(2):186-192.
APA	Gu, Jing.,Chen, Yuanyuan.,Guo, Hongsen.,Sun, Manluan.,Yang, Mingkun.,...&Deng, Jiaoyu.(2017).Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,49(2),186-192.
MLA	Gu, Jing,et al."Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 49.2(2017):186-192.