Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer
	Cheng, Ziyi 1; Choi, Namhyun 1; Wang, Rui 1; Lee, Sangyeop 1; Moon, Kyung Chul 2; Yoon, Soo-Young 2; Chen, Lingxin3 ; Choo, Jaebum 1
刊名	ACS NANO
	2017-05-01
卷号	11 期号:5 页码:4926-4933
关键词	SERS-based immunoassay multiplex detection prostate cancer free to total PSA ratio simultaneous detection
ISSN号	1936-0851
英文摘要	Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science
关键词[WOS]	TOTAL PSA ; BIOMARKER DETECTION ; SERS ; NANOPARTICLES ; MARKERS ; PROTEIN ; ASSAY ; DIFFERENTIATION ; NANOPROBES ; PERCENTAGE
收录类别	SCI
语种	英语
WOS记录号	WOS:000402498400061
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/22522]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea 2.Korea Univ, Dept Lab Med, Coll Med, Seoul 152854, South Korea 3.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Ziyi,Choi, Namhyun,Wang, Rui,et al. Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer[J]. ACS NANO,2017,11(5):4926-4933.
APA	Cheng, Ziyi.,Choi, Namhyun.,Wang, Rui.,Lee, Sangyeop.,Moon, Kyung Chul.,...&Choo, Jaebum.(2017).Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer.ACS NANO,11(5),4926-4933.
MLA	Cheng, Ziyi,et al."Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer".ACS NANO 11.5(2017):4926-4933.