Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2

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	Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2
	Jianbei Zhu ; Lei Xiong ; Yu B(俞飚) ; Jiarui Wu
刊名	Mol. Pharmacol.
	2005
卷号	68 期号:6 页码:1831-1838
ISSN号	0026-895X
其他题名	Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2
通讯作者	Jiarui Wu
英文摘要	OSW-1 is a new member of cholestane saponin family, which is cytotoxic against several types of malignant cells. We reported herein that OSW-1 induced apoptosis of mammalian cells in a concentration- and time-dependent manner. The drug-induced apoptosis was mediated through the mitochondrial pathway, involving the cleavage of Bcl-2. This drug-induced Bcl-2 cleavage in CHO cells could be suppressed either by dominant negative caspase-8 or by a caspase-8 inhibitor, suggesting that the Bcl-2 cleavage is dependent on caspase-8. In contrast, the Bcl-2 cleavage was independent of caspase-3 activity. The inhibition of caspase-8 activity also resulted in the reduction of apoptotic cells, indicating that Bcl-2 cleavage induced by caspase-8 promotes the progression of apoptosis. The involvement of the caspase-8 activity in the processes of the OSW-1 induced apoptosis was further examined by using caspase-8 deficient Jurkat T cells. It was found that the caspase-8 deficient cells were resistant to OSW-1 induced Bcl-2 cleavage or apoptosis. Furthermore, the small subunit of caspase-8 was found to interact with Bcl-2 as determined by yeast two-hybrid and co-immunoprecipitation assays. Overexpression of caspase-8 small subunit reduced the cleavage of Bcl-2 and inhibited the apoptosis induced by OSW-1. Taken together, these results demonstrate that OSW-1 is capable of inducing apoptosis in mammalian cells, in which the caspase-8 dependent cleavage of Bcl-2 plays an important role.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1124/mol.105.015826
语种	英语
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/19023]
专题	上海有机化学研究所_生命有机化学国家重点实验室
推荐引用方式 GB/T 7714	Jianbei Zhu,Lei Xiong,Yu B,et al. Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2[J]. Mol. Pharmacol.,2005,68(6):1831-1838.
APA	Jianbei Zhu,Lei Xiong,俞飚,&Jiarui Wu.(2005).Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2.Mol. Pharmacol.,68(6),1831-1838.
MLA	Jianbei Zhu,et al."Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2".Mol. Pharmacol. 68.6(2005):1831-1838.