Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation

	Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation
	Li N(李宁); Mao DB(毛德斌); Gong YX(龚一心); Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
刊名	Chinese Journal of Biomedical Engineering
	2015
卷号	24 期号:1 页码:40-46
中文摘要	LFA-1 and Mac-1, two β2integrin members constitutively expressed on neutrophils, mediate leukocyte recruitment cascade by binding to the same ligand of ICAM-1. The slow rolling and firm adhesion of leukocytes rely on LFA-1 while the cell crawling is dependent on Mac-1. We hypothesized that their distinct roles are likely attributed to the differences in the binding kinetics or in the diverse responses of outside-in and inside-out signaling. In this study, we compared the ICAM-1 binding features between soluble or membrane-expressed LFA-1 and Mac-1 with different affinity conformation using optical trap technique. Our data indicated that the affinity up-regulation from wide type(WT) to high affinity(HA) is off-rate dependent for LFA-1but on-rate dependent for Mac-1. The structural bases of this new finding were found to be consistent with our previous simulations. These results furthered our understanding in their function differences under shear flow.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/60641]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Li N,Mao DB,Gong YX,et al. Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation[J]. Chinese Journal of Biomedical Engineering,2015,24(1):40-46.
APA	Li N,Mao DB,Gong YX,Lv SQ,Zhang Y,&Long M.(2015).Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation.Chinese Journal of Biomedical Engineering,24(1),40-46.
MLA	Li N,et al."Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation".Chinese Journal of Biomedical Engineering 24.1(2015):40-46.