| 题名 | 大黄鱼幼鱼肠道菌群多样性分析及其两菌株几丁质酶和脂肪酶基因的克隆与表达; Analysis of intestinal microbiota of juvenile Pseudosciaena crocea (Richardson) and cloning, expression of chitinase and lipase gene in two strains from intestinal microbiota |
| 作者 | 许洁 |
| 答辩日期 | 2015 ; 2013 |
| 导师 | 艾春香 ; 周志刚 |
| 关键词 | 大黄鱼幼鱼 肠道菌群 高通量测序 传统培养法 几丁质酶基因 脂肪酶基因 克隆 juvenile Pseudosciaena crocea intestinal microbiota high-throughput sequencing traditional culture method chitinase gene lipase gene clone |
| 英文摘要 | 鱼类肠道中存在丰富的菌群，它们通过分泌酶类和产生活性物质参与宿主的生命活动，与机体的免疫和营养密切相关。本实验采用高通量测序技术（high-throughputsequencing）中的454焦磷酸测序技术（454pyrosequencing）分析分别摄食配合饲料或冰鲜鱼的大黄鱼（Pseudosciaenacrocea）幼鱼肠道壁和肠道内容物中的菌群；采用传统培养法筛选分离出大黄鱼幼鱼肠道内容物菌群，基于培养分离的幼鱼肠道内容物的菌株，采用FusionprimerandnestedintegratedPCR（FPNI-PCR）方法从柠檬酸杆菌P（Citrobactersp.P）克隆出几丁质酶基...; Numerous microorganism are found in intestine of fish. They contribute to the immunity and nutrition of host by secreting different enzymes and active substances. In this study, 454 pyrosequencing, a kind of high-throughput sequencing, was used for analysing the microbial community in intestinal tracts and contents of juvenile Pseudosciaena crocea fed with two different diets, formula feed and tra...; 学位：理学硕士; 院系专业：海洋与环境学院_海洋生物学; 学号：22420101151351 |
| 语种 | zh_CN |
| 出处 | http://210.34.4.13:8080/lunwen/detail.asp?serial=40837 |
| 内容类型 | 学位论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/96103]  |
| 专题 | 海洋地球－学位论文 |
| 推荐引用方式 GB/T 7714 | 许洁. 大黄鱼幼鱼肠道菌群多样性分析及其两菌株几丁质酶和脂肪酶基因的克隆与表达, Analysis of intestinal microbiota of juvenile Pseudosciaena crocea (Richardson) and cloning, expression of chitinase and lipase gene in two strains from intestinal microbiota[D]. 2015, 2013. |
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