| 小叶山葡萄化学成分及细胞毒活性研究; Chemical constituents and cytotoxicity assay research in small polar substances from Vitis thunbergii var. taiwaniana | |
| 姜超 ; 王文竹 ; 廖小军 ; 曾德泉 ; 林挺 ; 徐士兰 ; 曾锦章 ; 陈海峰 | |
| 2015-08-01 | |
| 关键词 | 小叶山葡萄 化学成分 茋类 细胞毒活性 Vitis thunbergii var.taiwaniana chemical constituents stilbene cytotoxicity |
| 英文摘要 | 该文对小叶山葡萄地上部分化学成分进行了研究,运用硅胶柱色谱,OdS中低压柱色谱,SEPHAdEX lH-20凝胶柱色谱,分析型和制备型HPlC从小叶山葡萄60%乙醇提取物大孔树脂95%乙醇-水洗脱部位分离得到12个化合物,利用高分辨质谱,核磁共振等波谱手段鉴定其结构分别为:(1)bETulInIC ACId,(2)2,2,2'-bIS(4-HydrOXyPHEnyl)PrOPAnE bIS(2,3-EPOXyPrOPyl)ETHEr,(3)ErIOdICTyOl,(4)TrAnS-ε-VInIfErIn,(5)(+)-CIS-ε-VInIfErIn,(6)kObOPHEnOl A,(7)AMPElOPSIn A,(8)nEPAlEnHEnOl,(9)CIS-MIyAbEnOl C,(10)CIS-VITISIn b,(11)CIS-gnETIn H和(12)(+)-HOPEAPHEnOl。化合物2,5,6,8,9,10,11均为首次从为葡萄属中分离得到的化合物,化合物3,7,12为首次从小叶山葡萄中分离得到的化合物。在作用浓度为50μMOl·l-1下,化合物6,7和11对MCf-7(乳腺癌细胞株)具有较明显的体外生长抑制作用,其抑制率分别为66.58%,57.16%,52.84%。; This article studied the chemical constituents from the aerial part of Vitis thunbergii var.taiwaniana.The 60% ethanol extract was eluted with 95% ethanol though HP-20 macroporous adsorption resin column.12 compounds,including( 1) betulinic acid,( 2) 2,2,2'-bis( 4-hydroxyphenyl) propane bis( 2,3-epoxypropyl) ether,( 3) eriodictyol,( 4) trans-ε-viniferin,( 5)( +)-cis-ε-viniferin,( 6) kobophenol A,( 7) ampelopsin A,( 8) nepalensinol B,( 9) cis-miyabenol C,( 10) cis-vitisin B,( 11) cis-gnetin H and( 12)( +)-hopeaphenol,were separated by using normal phase silica gel,ODS,Sephdadex LH-20 column chromatographies and semi-preparative or preparative HPLC.Compounds 2,5,6,8,9,10,11 were separated from the genus Vitis for the first time and compounds 3,7,12 were separated from Vitis thunbergii var.taiwaniana for the first time.At a concentration of 50 μmol·L-1,compound6,7 and 11 showed strong cytotoxicity against MCF-7 cell lines with the inhibition rate of 66.58%,57.16%,52.84%,respectively.; 国家自然科学基金青年基金项目(81202419) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/123605]  |
| 专题 | 药学院－已发表论文 |
| 推荐引用方式 GB/T 7714 | 姜超,王文竹,廖小军,等. 小叶山葡萄化学成分及细胞毒活性研究, Chemical constituents and cytotoxicity assay research in small polar substances from Vitis thunbergii var. taiwaniana[J],2015. |
| APA | 姜超.,王文竹.,廖小军.,曾德泉.,林挺.,...&陈海峰.(2015).小叶山葡萄化学成分及细胞毒活性研究.. |
| MLA | 姜超,et al."小叶山葡萄化学成分及细胞毒活性研究".(2015). |
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