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	高效液相色谱法测定人血清中伏立康唑的质量浓度; Determination of Serum Voriconazole Concentration by HPLC
	张燕青 ; 林雪玉 ; 朱金平 ; 费燕
	2014-12-05
关键词	伏立康唑 高效液相色谱法 血药浓度 voriconazole HPLC blood drug concentration
英文摘要	目的建立测定人血清中伏立康唑浓度的高效液相色谱法,用于临床该药的治疗药物浓度监测。方法采用乙腈沉淀处理血清样品,采用高效液相色谱紫外检测法测定。以硝西泮为内标,色谱柱为HyPErSIl OdS柱(200 MMx4.6 MM,5μM),流动相为25 MMOl/l磷酸二氢钾缓冲液-乙腈(32∶68),流速为1.0 M l/MIn,柱温为35℃,波长为256 nM。结果伏立康唑质量浓度在0.3~12μg/M l范围内与峰面积比线性关系良好(r=0.999 3),低、中、高质量浓度回收率分别为(105.0±3.6)%,(101.9±3.3)%,(101.1±3.4)%;日内、日间精密度的rSd均小于5%,定量限为0.3μg/M l。结论该方法简便快速、准确,适用于伏立康唑血药浓度的临床监测。; Objective To establish a HPLC method to determine the concentration of voriconazole in human plasma for its clinical application in the curative drug concentration monitoring.Methods The serum sample was performed the precipitation treatment by actetonitrile, then HPLC- UV was adopted for detection.With nitrazepan as the internal standard, the chromatographic column was the Hypersil ODS columm( 200.0 mm × 4.6 mm, 5 μm), the mobile phase was 25 mmol / L potassium dihydrogen phosphate buffer solution-acetonitrile( 32 ∶ 68).The detection wavelength was at 256 nm with a flow rate of 1 m L / min and the column temperature of 35 ℃.Results The concentration of voriconazole in the range of 0.3-12 μg / mL( r = 0.999 3) showed the good linearity.The recovery rates of low, middle and high concentrations were( 105.0 ± 3.6) %,( 101.9 ± 3.3) % and( 101.1 ± 3.4) %, respectively.The intra- day and inter- day RSD were both less than 5%.The limit of quantitation was 0.3 μg / m L.Conclusion The established method is fast,accurate and suitable for the determination of blood voriconazole concentration.
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123469]
专题	药学院－已发表论文
推荐引用方式 GB/T 7714	张燕青,林雪玉,朱金平,等. 高效液相色谱法测定人血清中伏立康唑的质量浓度, Determination of Serum Voriconazole Concentration by HPLC[J],2014.
APA	张燕青,林雪玉,朱金平,&费燕.(2014).高效液相色谱法测定人血清中伏立康唑的质量浓度..
MLA	张燕青,et al."高效液相色谱法测定人血清中伏立康唑的质量浓度".(2014).

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