| Hg~(2+)、Cu~(2+)、Zn~(2+)、Cd~(2+)胁迫下罗非鱼离体肝脏中GSH和GST的响应; Responses of GSH and GST in liver of Mossambica tilapia under Hg~(2+),Cu~(2+),Zn~(2+) and Cd~(2+) stress in Vitro | |
| 肖丹 ; 王海燕 ; 韩大雄 | |
| 2014-06-20 | |
| 关键词 | 罗非鱼 重金属 谷胱甘肽 谷胱甘肽-S-转移酶 肝上清液 Mossambica tilapia heavy metals GSH GST hepatic supernatant |
| 英文摘要 | 以罗非鱼(MOSSAMbICA TIlAPIA)为实验材料,研究了不同浓度的4种金属离子(Hg2+、Cu2+、zn2+、Cd2+)体外暴污对其肝脏上清液中谷胱甘肽(gSH)含量和谷胱甘肽-S-转移酶(gST)活性的影响,以探讨其用于污染暴露的生物标记的可能性。结果表明:暴露于上述4种金属离子的罗非鱼肝上清液中,gSH含量均为低浓度升高,高浓度降低。Hg2+、Cu2+、zn2+处理组均在100 nMOl/l时,gSH含量上升至最大值,与对照组相比,均差异极显著(P≤0.01)。gST的活性则均随着4种金属离子浓度的升高总体呈下降趋势,4个处理组gST活性的变化在整个浓度范围内的剂量-效应关系均存在一定的线性规律。实验结果表明鱼类肝上清液中gSH含量和gST活性的变化很有潜力运用于环境监测系统中,成为环境中金属离子污染的早期生物预警标志物。; This paper approached the feasible of using the GSH content and GST activity in Mossambica tilapia liver supernatant as the biological indicator of 4 heavy metals(Hg2 +,Cu2 +,Zn2 +,Cd2 +) toxic effect on this fish.Results indicated that the GSH content was elevated by low concentration of the 4 heavy metals while reduced by high dosage of the 4 pollutants.When the concentration of Hg2 +,Cu2 +,Zn2 +was 100 nmol / L,the GSH content increased significantly and reached the maximum(p≤0.01).The activity of GST decreased continuously as the concentration of 4 heavy metals increased and there was a certain dose-response linear relationship in the whole assayed concentrations.The results indicated that the change of GSH content and GST activity in the fish liver supernatant could be a potential biomarker,which could be used in environmental monitoring system to reflect the early heavy metal ion pollution.; 海洋公益性项目(201105013); 福建省重大专项/专题项目(2011YZ0001-1); 国家海洋局第三海洋研究所基本科研业务费专项基金资助项目(201221); 福建省自然科学基金项目(2013J01386) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/123455]  |
| 专题 | 药学院－已发表论文 |
| 推荐引用方式 GB/T 7714 | 肖丹,王海燕,韩大雄. Hg~(2+)、Cu~(2+)、Zn~(2+)、Cd~(2+)胁迫下罗非鱼离体肝脏中GSH和GST的响应, Responses of GSH and GST in liver of Mossambica tilapia under Hg~(2+),Cu~(2+),Zn~(2+) and Cd~(2+) stress in Vitro[J],2014. |
| APA | 肖丹,王海燕,&韩大雄.(2014).Hg~(2+)、Cu~(2+)、Zn~(2+)、Cd~(2+)胁迫下罗非鱼离体肝脏中GSH和GST的响应.. |
| MLA | 肖丹,et al."Hg~(2+)、Cu~(2+)、Zn~(2+)、Cd~(2+)胁迫下罗非鱼离体肝脏中GSH和GST的响应".(2014). |
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