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	静脉注射IL-19重组质粒对大鼠实验性自身免疫性心肌炎的治疗作用; Effect of intravenous injecting plasmid encoding interleukin-19-IgG on experimental autoimmune myocarditis in rats
	常贺 ; 赵法允 ; 王焱 ; 李刚 ; 张乐 ; 邹军
	2015-04-15
关键词	白细胞介素19 自身免疫性心肌炎 Interleukin-19 Autoimmune myocarditis
英文摘要	目的:评估静脉注射白细胞介素19(InTErlEukIn-19,Il-19)重组质粒对大鼠实验性自身免疫性心肌炎(EXPErIMEnTAl AuTOIMMunE MyOCArdITIS,EAM)的治疗作用。方法:将猪心室肌球蛋白与等体积完全弗氏免疫佐剂充分混匀后,双足皮下注射制作大鼠EAM模型,免疫后第6天应用静脉注射方法将Il-19重组质粒导入体内,第17天行心脏超声检查后处死大鼠,检测心脏重量与体重比值、病理学评估、心肌炎面积率;实时荧光定量PCr检测心衰标记物心房钠尿肽(ATrIAl nATrIurETIC PEPTIdE,AnP)、脑钠尿肽(brAIn nATrIurETIC PEPTIdE,bnP)的表达水平,进一步检测心肌相关炎症因子Il-18、Il-1β、Il-12P35和Ifn-γ的表达水平。结果:Il-19重组质粒导入组的大鼠心功能得到显著改善;心脏重量体重比、心肌炎面积率、AnP、bnP的表达均较模型组明显下降;相关炎症细胞因子的表达也显著降低。结论:应用静脉注射法进行Il-19重组质粒体内导入治疗,明显抑制大鼠实验性自身免疫性心肌炎的炎症反应,减轻了心肌损伤从而改善心功能。; AIM:To evaluate the effect of intravenous injecting plasmid encoding interleukin-19-Ig G on experimental autoimmune myocarditis(EAM) in rats.METHODS:Cardiac myosin was emulsified with equal volume of complete Freund's adjuvant.The animal model of EAM was established by injecting with the preparation in both footpads of the Lewis rats.The rats were intravenously injected with the plasmid encoding IL-19-Ig G on day 6.Echocardiography was performed before the rats were sacrificed on day 17.The effect of IL-19-Ig G plasmid injection was evaluated by measuring the heart weight / body weight,myocarditis area,relative expression levels of atrial natriuretic peptide(ANP) and brain natriuretic peptide(BNP) in the hearts.The mRNA expression levels of related cytokines including IL-18,IL-1β,IL-12p35 and IFN-γ were detected.RESULTS:The rats in model group showed significant myocardial damage and a decrease in the left ventricular functions.The rats in the treatment group injected with IL-19-Ig G plasmid showed an improvement of the cardiac functions.The ratio of heart weight / body weight,the area of myocarditis and the mRNA levels of ANP and BNP were significantly lower in IL-19-Ig G treatment group than those in model group.The mRNA levels of IL-18,IL-1β,IL-12p35 and IFN-γ were also significantly decreased in IL-19-Ig G treatment group.CONCLUSION:Intravenous injection of plasmid encoding IL-19-Ig G effectively prevents the development of the left ventricular remodeling and myocardial damage in EAM rats.; 国家自然科学基金资助项目(No.81270294); 福建省卫生系统中青年骨干人才项目(No.2013-ZQN-ZD-37); 福建省自然科学基金资助项目(No.2012J01415)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123794]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	常贺,赵法允,王焱,等. 静脉注射IL-19重组质粒对大鼠实验性自身免疫性心肌炎的治疗作用, Effect of intravenous injecting plasmid encoding interleukin-19-IgG on experimental autoimmune myocarditis in rats[J],2015.
APA	常贺,赵法允,王焱,李刚,张乐,&邹军.(2015).静脉注射IL-19重组质粒对大鼠实验性自身免疫性心肌炎的治疗作用..
MLA	常贺,et al."静脉注射IL-19重组质粒对大鼠实验性自身免疫性心肌炎的治疗作用".(2015).

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