| 花生四烯酸乙醇胺对大鼠心肌细胞L型钙电流的影响; Effect of anandamide on L-type calcium current in cardiomyocytes of rats | |
| 周丽 ; 李威 ; 唐明 | |
| 2013 | |
| 关键词 | 花生四烯酸乙醇胺 全细胞膜片钳技术 心肌细胞 L型钙电流 激活 失活 anandamide patch-clamp technique car diomyocytes L-type calcium current activation inac tivation |
| 英文摘要 | 目的研究AnAndAMIdE(花生四烯酸乙醇胺)对单个大鼠心肌细胞l型钙电流(I CA-l)的影响。方法应用全细胞膜片钳技术记录I CA-l,并观察AnAndAMIdE对该电流的作用。结果 (1)AnAndAMIdE浓度依赖性地减小I CA-l,其IC50值为(1.3±0.3)μMOl·l-1。(2)1μMOl·l-1AnAndAMIdE可明显使稳态失活曲线左移,失活中点电压(V1/2)由(-34.4±0.2)MV变为(-42.2±0.6)MV,k值由(5.7±0.2)变为(6.4±0.4),表明AnAndAMIdE改变了钙通道失活的电压依赖性。AnAndAMIdE 1μMOl·l-1对稳态激活曲线无明显影响。结论 AnAndAMIdE可使稳态失活曲线左移而剂量依赖性地减小I CA-l。; Aim To investigate the effect of anandamide on L-type Ca2 +current(I Ca-L) in rat ventricular myocytes.Methods I Ca-L was recorded by patchclamp technique in whole-cell configuration.Results(1) Anandamide dose-dependently decreased I Ca-L.The value of IC 50 was(1.3 ± 0.3) μmol·L-1.(2) 1 μmol·L-1anandamide shifted the steady-state inactivation curve to the left and changed half inactivation potential(V 1 /2) from(-34.4 ±0.2) mV to(-42.2 ± 0.6) mV,and the value of κ changed from(5.7 ± 0.2) to(6.4 ± 0.4),indicating that anandamide modified the voltage-dependent inactivation of calcium channel.Anandamide did not affect the steady-state activation curve of I Ca-L.Conclusion These effects suggest that anandamide decreases I Ca-L by shifting its inactivation curve to the left.; 国家自然科学基金资助项目(No81000560) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/123680]  |
| 专题 | 医学院－已发表论文 |
| 推荐引用方式 GB/T 7714 | 周丽,李威,唐明. 花生四烯酸乙醇胺对大鼠心肌细胞L型钙电流的影响, Effect of anandamide on L-type calcium current in cardiomyocytes of rats[J],2013. |
| APA | 周丽,李威,&唐明.(2013).花生四烯酸乙醇胺对大鼠心肌细胞L型钙电流的影响.. |
| MLA | 周丽,et al."花生四烯酸乙醇胺对大鼠心肌细胞L型钙电流的影响".(2013). |
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