Protective effects of mesenchymal stromal cells on adriamycin-induced minimal change nephrotic syndrome in rats and possible mechanisms

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	Protective effects of mesenchymal stromal cells on adriamycin-induced minimal change nephrotic syndrome in rats and possible mechanisms
	Guo, Junqi ; Zou, Yuhua ; Wu, Zhixian ; Wu, Weizhen ; Xu, Zizhong ; Hu, Heyi ; Huang, Lianghu ; Dong, Huiyue ; Chen, Jin ; Lu, Jun ; Fu, Yunfen ; Wang, Jin ; Ma, Yujie ; Chen, Xiaowen ; He, Fuqiang ; Yang, Shunliang ; Liao, Lianming ; Chen, Jian ; Zheng, Feng ; Tan, Jianming ; Guo JQ(郭君其) ; Wu WZ(吴卫真) ; Lu J(路君) ; Yang SL(杨顺良)
刊名	http://dx.doi.org/10.1016/j.jcyt.2013.08.002
	2014
关键词	CHRONIC-RENAL-FAILURE ACUTE KIDNEY INJURY STEM-CELLS PODOCYTE INJURY DIABETIC-NEPHROPATHY OXIDATIVE STRESS SLIT DIAPHRAGM GROWTH-FACTOR T-CELLS MODEL
英文摘要	Fujian Science and Technology Innovation Platform Construction Plan [2008J10060, 2010Y2006]; Technology Innovation Projects [09MA093]; Fujian Natural Science Foundation [2012J01404]; Fujian Science and Technology Project Foundation [2012Y5006]; Background aims. Minimal change nephrotic syndrome is the most frequent cause of nephrotic syndrome in childhood. Current treatment regimes, which include glucocorticoid hormones and immunosuppressive therapy, are effective and have fast response. However, because of the side effects, long treatment course, poor patient compliance and relapse, novel approaches for the disease are highly desired. Methods. The adriamycin-induced nephrotic rat model was established. Rats were allocated to a model group, a prednisone group or mesenchymal stromal cell (MSC) group. Clinical parameters in each treatment group were determined at 2 weeks, 4 weeks and 8 weeks. The messenger RNA (mRNA) levels of synaptopodin, p21 and monocyte chemoattractant protein-1 were determined through the use of quantitative real-time polymerase chain reaction. Protein levels were determined by means of Western blot or enzyme-linked immunosorbent assay. Podocytes were isolated and apoptotic rate after adriamycin with or without MSC treatment was analyzed by means of flow cytometry. Results. MSC intervention improved renal function as assessed by urinary protein, blood creatinine and triglyceride levels. MSC intervention reduced adriamycin-induced renal tissue damage visualized by immunohistochemistry and light and electron microscopic analysis and reduced adriamycin-induced podocyte apoptosis. After MSC intervention, mRNA and protein levels of synaptopodin and p21 in renal cortex were significantly increased. MSCs also restored synaptopodin mRNA and protein expression in isolated podocytes. In addition, monocyte chemoattractant protein-1 mRNA in renal cortex and protein level in serum of the MSC treatment group were significantly decreased compared with that in the adriamycin-induced nephropathy model group. Conclusions. Our data indicate that MSCs could protect rats from adriamycin-induced minimal change nephrotic syndrome, and the protective effects of MSCs are mediated through multiple actions.
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93643]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Guo, Junqi,Zou, Yuhua,Wu, Zhixian,et al. Protective effects of mesenchymal stromal cells on adriamycin-induced minimal change nephrotic syndrome in rats and possible mechanisms[J]. http://dx.doi.org/10.1016/j.jcyt.2013.08.002,2014.
APA	Guo, Junqi.,Zou, Yuhua.,Wu, Zhixian.,Wu, Weizhen.,Xu, Zizhong.,...&杨顺良.(2014).Protective effects of mesenchymal stromal cells on adriamycin-induced minimal change nephrotic syndrome in rats and possible mechanisms.http://dx.doi.org/10.1016/j.jcyt.2013.08.002.
MLA	Guo, Junqi,et al."Protective effects of mesenchymal stromal cells on adriamycin-induced minimal change nephrotic syndrome in rats and possible mechanisms".http://dx.doi.org/10.1016/j.jcyt.2013.08.002 (2014).