EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells

CORC > 厦门大学 > 医学院－已发表论文

	EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells
	Chen, Peijie ; Huang, Yan ; Zhang, Bo ; Wang, Qiuquan ; Bai, Peiming ; Chen PJ(陈培杰) ; Zhang B(张博) ; Wang QQ(王秋泉) ; Bai PM(白培明)
刊名	http://dx.doi.org/10.3892/ol.2014.2093
	2014
关键词	RECEPTOR TYROSINE KINASE OVARIAN-CANCER OVEREXPRESSION EXPRESSION CARCINOMA GENE ANGIOGENESIS METASTASIS GROWTH ECK
英文摘要	National Natural Science Foundation of China [21035006]; EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit-8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid-mediated pcDNA3.1(+)-EphA2, markedly enhanced the cell growth and invasion in vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of Eph.A2 was significantly associated with Gleason grade, total prostate-specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EptLA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer.
语种	英语
出版者	SPANDIDOS PUBL LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93594]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Chen, Peijie,Huang, Yan,Zhang, Bo,et al. EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells[J]. http://dx.doi.org/10.3892/ol.2014.2093,2014.
APA	Chen, Peijie.,Huang, Yan.,Zhang, Bo.,Wang, Qiuquan.,Bai, Peiming.,...&白培明.(2014).EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells.http://dx.doi.org/10.3892/ol.2014.2093.
MLA	Chen, Peijie,et al."EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells".http://dx.doi.org/10.3892/ol.2014.2093 (2014).