Lipoxin A(4) regulates expression of the estrogen receptor and inhibits 17 beta-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells

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	Lipoxin A(4) regulates expression of the estrogen receptor and inhibits 17 beta-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells
	Chen, Shuo ; Wu, Rong-Feng ; Su, Lin ; Zhou, Wei-Dong ; Zhu, Mao-Bi ; Chen, Qiong-Hua ; Chen QH(陈琼华)
刊名	http://dx.doi.org/10.1016/j.fertnstert.2014.03.029
	2014
关键词	OVARIAN ENDOMETRIOSIS IN-VIVO ER-BETA INFLAMMATION ALPHA MODEL SUPPRESSES RESOLUTION GENES MICE
英文摘要	Science and Technology Planning Project of Xiamen City, People's Republic of China [3502 Z 20134002]; Natural Science Foundation of Fujian Province [2013D001]; Objective: To study the role of lipoxin A(4) (LXA(4)) in endometriosis. Design: Molecular analysis in human samples and primary human endometriotic stromal cells (ESCs). Setting: University hospital. Patient(s): Forty-nine premenopausal women (30 patients with endometriosis and 19 controls). Intervention(s): Normal and ectopic endometrial biopsies obtained during surgery performed during the proliferative phase of the menstrual cycle; ESCs used for in vitro studies. Main Outcome Measure(s): Levels of LXA(4) measured by enzyme-linked immunosorbent assay (ELISA); mRNA levels of the estrogen receptor (ER), progestogen receptor (PR), tumor necrosis factor alpha (TNF-alpha), and interleukin 6 (IL-6) quantified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR); and p38 mitogen-activated protein kinase (p38 MAPK) phosphorylation evaluated by Western blotting. Result(s): The LXA(4) expression level decreased in ectopic tissue as well as ER alpha and PR, although the expression of ER beta increased in ectopic endometrium compared with the controls. Investigations with correlation analysis revealed the expression of LXA(4) was positively correlated with ER alpha and negatively correlated with ER beta in vivo. Moreover, administering LXA(4) could augment ER beta expression in ESCs and inhibit the 17 beta-estradiol-induced phosphorylation of p38 MAPK very likely through ER beta. Conclusion(s): Our findings indicate that LXA(4) regulates ER beta expression and inhibits 17 beta-estradiol-induced phosphorylation of p38 MAPK, very likely through ER beta in ESCs. (C)2014 by American Society for Reproductive Medicine.
语种	英语
出版者	ELSEVIER SCIENCE INC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93588]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Chen, Shuo,Wu, Rong-Feng,Su, Lin,et al. Lipoxin A(4) regulates expression of the estrogen receptor and inhibits 17 beta-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells[J]. http://dx.doi.org/10.1016/j.fertnstert.2014.03.029,2014.
APA	Chen, Shuo.,Wu, Rong-Feng.,Su, Lin.,Zhou, Wei-Dong.,Zhu, Mao-Bi.,...&陈琼华.(2014).Lipoxin A(4) regulates expression of the estrogen receptor and inhibits 17 beta-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells.http://dx.doi.org/10.1016/j.fertnstert.2014.03.029.
MLA	Chen, Shuo,et al."Lipoxin A(4) regulates expression of the estrogen receptor and inhibits 17 beta-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells".http://dx.doi.org/10.1016/j.fertnstert.2014.03.029 (2014).