Carboxylated single-walled carbon nanotubes induce an inflammatory response in human primary monocytes through oxidative stress and NF-kappa B activation

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	Carboxylated single-walled carbon nanotubes induce an inflammatory response in human primary monocytes through oxidative stress and NF-kappa B activation
	Ye, Shefang ; Ye SF(叶社房) ; Zhang, Honggang ; Wang, Yifang ; Jiao, Fei ; Lin, Cuilin ; Zhang, Qiqing ; Zhang QQ(张其清)
刊名	http://dx.doi.org/10.1007/s11051-011-0368-1
	2011-09
关键词	LUNG EPITHELIAL-CELLS IN-VITRO DNA-DAMAGE PARTICLES APOPTOSIS NANOPARTICLES EXPRESSION TOXICITY ALPHA MICE
英文摘要	National Natural Science Foundation of China [30901175]; Science and Technology Innovation Project of Fujian Province for Young Scientific Researchers, China [2008F3097]; A mechanistic understanding of interactions between carbon nanotubes (CNTs) and living systems has become imperative owing to the growing nanomedicine applications and the mounting societal concerns on nanosafety. The addition of different chemical groups leads to a significant change in the properties of CNTs, and the resulting functionalized CNTs are generating great interest in many biological applications, such as biosensors and transporters. This study aimed to assess the toxicity exhibited by carboxylic acid functionalized single-walled CNTs (SWCNTs) (with a diameter of 1-2 nm and mean length of 500 nm) and to elucidate possible molecular mechanisms underlying the biological effects of carboxylated SWCNTs in human primary monocytes. The results demonstrated that carboxylated SWCNTs were cytotoxic, triggering apoptosis and G(2)/M phase arrest in human primary monocytes. Flow cytometric and confocal microscopic analysis indicated that internalized carboxylated SWCNTs were mainly accumulated in the cytoplasm. Exposure of human primary monocytes to carboxylated SWCNTs led to interleukin-8 (IL-8) and interleukin-6 (IL-6) expression, reactive oxygen species (ROS) production, and nuclear factor-kappa B (NF-kappa B) activation in human primary monocytes. Pretreatment of human primary monocytes with antioxidants or NF-kappa B-specific inhibitor before exposure to carboxylated SWCNTs significantly abolished carboxylated SWCNTs-induced IL-8 and IL-6 expression. These results provide novel insights into the carboxylated SWCNTs-mediated chemokine induction and inflammatory responses in vitro.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/71414]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Ye, Shefang,Ye SF,Zhang, Honggang,et al. Carboxylated single-walled carbon nanotubes induce an inflammatory response in human primary monocytes through oxidative stress and NF-kappa B activation[J]. http://dx.doi.org/10.1007/s11051-011-0368-1,2011.
APA	Ye, Shefang.,叶社房.,Zhang, Honggang.,Wang, Yifang.,Jiao, Fei.,...&张其清.(2011).Carboxylated single-walled carbon nanotubes induce an inflammatory response in human primary monocytes through oxidative stress and NF-kappa B activation.http://dx.doi.org/10.1007/s11051-011-0368-1.
MLA	Ye, Shefang,et al."Carboxylated single-walled carbon nanotubes induce an inflammatory response in human primary monocytes through oxidative stress and NF-kappa B activation".http://dx.doi.org/10.1007/s11051-011-0368-1 (2011).