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	Bear Bile Inhibits the Immunosuppression Activity of Hepatic Stellate Cells In Vivo
	Zhou, Chang-Sheng ; Yin, Zhen-Yu ; Zhao, Wen-Xiu ; Zhang, Lei ; You, Jun-Yong ; Su, Wei-Xue ; Fan, Jia ; Wang, Xiao-Min ; Wang XM(王效民)
	2012-07
关键词	Bear bile Hepatic stellate cells Immune Inhibition Chronic liver disease
英文摘要	Background/Aims: In the injured liver, hepatic stellate cells (HSCs) induce immunosuppression activity and thus participate in the pathogenesis of liver disease, including HCC. Therefore, finding new drugs to inhibit their immunosuppression activity is necessary. This study tests whether bear bile can affect the immunosuppression activities of HSCs. Methodology: The mice were gavaged with bear bile for 4 weeks. The expression of HSCs was detected through desmin and a-smooth muscle antibody immunohistochemistry. HSCs were isolated from these mice liver and then cultured with T cells in a mixed leukocyte reaction for 3 days. Stellate cell surface makers, T-cell apoptosis, regulatory T cells and the ability of T cells to kill hepatocellular carcinoma were determined via flow cytometry. Cytokines were determined by a mouse cytokine array panel and T-cell proliferation was determined through a BrdU kit. Results: Bear bile decreased HSCs and their surface molecules, and affected cytokine secretion. Interestingly, HSCs from the mice gavaged with bear bile promoted T-cell proliferation, inhibited T-cell apoptosis, decreased CD4(+)CD25(+)Foxp3(+) regulatory T cells and enhanced the activation of T cells killing hepatocellular carcinoma. Conclusions: Bear bile can inhibit the immunosuppression activity of HSCs and enhance immune response especial anti-tumor immune response.; National Key Sci-Tech Special Project of China [2008ZX10002-019]; National Natural Science Foundation of China [30872484]
语种	英语
出版者	H G E UPDATE MEDICAL PUBLISHING S A
内容类型	期刊论文
源URL	[http://dx.doi.org/10.5754/hge10637]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhou, Chang-Sheng,Yin, Zhen-Yu,Zhao, Wen-Xiu,et al. Bear Bile Inhibits the Immunosuppression Activity of Hepatic Stellate Cells In Vivo[J],2012.
APA	Zhou, Chang-Sheng.,Yin, Zhen-Yu.,Zhao, Wen-Xiu.,Zhang, Lei.,You, Jun-Yong.,...&王效民.(2012).Bear Bile Inhibits the Immunosuppression Activity of Hepatic Stellate Cells In Vivo..
MLA	Zhou, Chang-Sheng,et al."Bear Bile Inhibits the Immunosuppression Activity of Hepatic Stellate Cells In Vivo".(2012).

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