Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

	Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry
	You, JM ; Shan, YC ; Zhen, L ; Zhang, L ; Zhang, YK
刊名	analytical biochemistry
	2003-02-01
卷号	313 期号:1 页码:17-27
关键词	derivatization HPLC LC/MS amino acids 2-(9-carbazole)-ethyl chloroformate wool beer
产权排序	1;1
英文摘要	a new method for the sensitive determination of amino acids and peptides using the tagging reagent 2-(9-carbazole)-ethyl chloroformate (ceoc) with fluorescence (fl) detection has been developed. identification of derivatives was carried out by liquid chromotography mass spectrometry. the chromophore in the 2-(9-fluorenyl)-ethyl chloroformate (fmoc) reagent was replaced by carbazole, which resulted in a sensitive fluorescence lerivatizing agent ceoc. ceoc can easily and quickly label peptides and amino acids. derivatives are stable enough to be efficiently analyzed by high-performance liquid chromatography. studies on derivatization demonstrate excellent derivative yields over the ph range 8.8-10.0. maximal yields close to 100% are observed with three- to fourfold molar reagent excess. derivatives exhibit strong fluorescence and allow direct injection of the reaction mixture with no significant disturbance from the major fluorescent reagent degradation by-products, such as 2(9-carbazole)-ethanol and bis-(2-(9-carbazole)-ethyl) carbonate. in addition, the detection responses for ceoc derivatives are compared to those obtained with fmoc. the ratios ac(ceoc)/ac(fmoc) = 1.00-1.82 for fluorescence (fl) response and ac'(ceoc)/ac'(fmoc) = 1.00-1.21 for ultraviolet (uv) response are observed (here, ac and ac' are, respectively, fl and uv f response). separation of the derivatized peptides and amino acids has been optimized on a hypersil bds c18 column. excellent linear responses are observed. this method was used successfully to analyze protein hydrolysates from wool and from direct-derivatized beer. (c) 2003 elsevier science (usa). all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	9-fluorenylmethyl chloroformate ; chromatographic determination ; derivatization ; proteins ; isothiocyanate
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000181093100003
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/83473]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China
推荐引用方式 GB/T 7714	You, JM,Shan, YC,Zhen, L,et al. Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry[J]. analytical biochemistry,2003,313(1):17-27.
APA	You, JM,Shan, YC,Zhen, L,Zhang, L,&Zhang, YK.(2003).Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry.analytical biochemistry,313(1),17-27.
MLA	You, JM,et al."Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry".analytical biochemistry 313.1(2003):17-27.