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	桑叶黄酮抑制酪氨酸酶活性的动力学研究; Study of kinetics with respect to action of flavonoid from mulberry leaves on inhibition of activity of tyrosinase
	付建红 ; 祁瑞 ; 郑静 ; 周晨婕 ; 陈清西
	2014-10-22
关键词	美白化妆品添加剂 桑叶黄酮 酪氨酸酶 抑制机理 whitening cosmetics additive flavonoid from mulberry leaves tyrosinase inhibitory mechanism
英文摘要	采用超声波辅助法,以体积分数为60%的乙醇水溶液从新疆桑叶粉末中提取桑叶黄酮。以l-多巴为底物,采用酶动力学方法研究该提取物对酪氨酸酶的抑制作用。实验结果表明,桑叶黄酮提取物对酪氨酸酶有较强的抑制作用,其IC50为0.2 g·l-1。其对酪氨酸酶的抑制作用表现为可逆效应,抑制类型为混合型抑制,对游离酶的抑制常数(kI)和对酶-底物络合物的抑制常数(kIS)分别为366和873 Mg·l-1。dPPH实验显示新疆桑叶黄酮提取物具有一定的抗氧化作用,对dPPH自由基氧化作用相对活性的IC50为25 Mg·l-1,与VC效果相当。; Flavonoid was extracted from powder of mulberry leaves planted in Xinjiang with 60%( vol) ethanol aqueous solution under assistance of ultrasonic wave.Adopting enzymatic kinetic method,inhibitory action of the extract on tyrosinase activity was studied using L- dopa as the substrate.Results showed that the flavonoid extract displays rather strong inhibitory action on activity of tyrosinase.Its IC50 was estimated as 0.2 g·L- 1.The inhibition mechanism was studied and it was found that the reaction is reversible and type of the inhibition belongs to the mixed type,with the inhibition equilibrium constants for inhibitor binding with free enzyme KI and with enzyme- substrate complex KISwere determined as 366 and 873 mg·L- 1respectively.The DPPH( 1,1-diphenyl-2-picrylhydrazyl) radical test results showed that the flavonoid extract has the antioxidant activity to a certain extent.The IC50 is 25 mg·L- 1that is similar to vitamin C.; 新疆维吾尔自治区高校科研计划科学研究重点资助项目(XJEDU2012I30); 新疆师范大学校级重点学科招标课题资助项目(XJMB-1)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119267]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	付建红,祁瑞,郑静,等. 桑叶黄酮抑制酪氨酸酶活性的动力学研究, Study of kinetics with respect to action of flavonoid from mulberry leaves on inhibition of activity of tyrosinase[J],2014.
APA	付建红,祁瑞,郑静,周晨婕,&陈清西.(2014).桑叶黄酮抑制酪氨酸酶活性的动力学研究..
MLA	付建红,et al."桑叶黄酮抑制酪氨酸酶活性的动力学研究".(2014).

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