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	辐照处理对蟹类过敏原(原肌球蛋白)性质的影响; The Effects of Irradiation on the Allergenicity of Crab Tropomyosins
	刘光明 ; 王玉松 ; 黄园园 ; 曹敏杰 ; 张凌晶 ; 苏文金
	2009
关键词	蟹类 原肌球蛋白 辐照处理 免疫检测 模拟胃液消化 crab tropomyosin radiation treatment immunoassay simulated gastric fluid digestion
英文摘要	食物过敏是当前食品安全领域较为突出的问题,辐照作为食物过敏原的加工处理方法之一,引起了学者的重视.作者对中华绒鳌蟹和三疣梭子蟹的主要过敏原(原肌球蛋白)进行了辐照处理,并分析了辐照处理对原肌球蛋白性质的影响.十二烷基磺酸钠-聚丙烯酰胺凝胶电泳结果显示,部分纯化的蟹类原肌球蛋白经7 kgy辐照处理后出现明显降解,10 kgy辐照处理后完全降解;蟹类过敏者血清的免疫印迹分析结果显示,部分纯化原肌球蛋白经3--7 kgy辐照处理后的过敏原性无明显变化,而10 kgy辐照处理后的过敏原性降低;兔抗锯缘青蟹原肌球蛋白抗体的免疫胶体金层析检测结果证实部分纯化原肌球蛋白经10 kgy辐照处理后的抗原性为阴性.但是,3--10 kgy辐照处理对整蟹原肌球蛋白及其过敏原性无明显影响,模拟胃液消化反应结果也表明3--10 kgy辐照处理对整蟹原肌球蛋白的酶解消化特性无明显影响.; Food allergy is a prominent issue in the field of food safety.Irradiation treatment of food allergens has aroused extensive attention in the world.This study focused on the effects on the characteristic of the allergen(tropomyosins) from Chinese mitten crab and Swimming crab through irradiation.Analysis of SDS-PAGE showed that partially purified tropomyosins obviously degraded after treated with 7 kGy dose while almost degraded after 10 kGy treatment.Analysis of Western-Blotting with crab allergenic patients' sera indicated that the allergenicity of partially purified tropomyosins that treated by 10 kGy irradiation have an obvious decrease while that of partially purified tropomyosins treated by 3--7 kGy irradiation have slight differences.The results of the gold immunochromatography assay with animal antibody indicated that the antigenicity of partially purified tropomyosins was negative after treatment with 10 kGy irradiation.On the other hand,there was little effect on the allergenicity of tropomyosins from the entire Chinese mitten crab and the entire Swimming crab through irradiation.The simulating digestion experiment of gastric fluid also showed that there was little effect on the digestive stability of the tropomyosins from the entire crab through irradiation.; 国家自然科学基金(30571450;30871947);福建省科技计划重点项目(2006I0023;2006F5064)资助
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/118872]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	刘光明,王玉松,黄园园,等. 辐照处理对蟹类过敏原(原肌球蛋白)性质的影响, The Effects of Irradiation on the Allergenicity of Crab Tropomyosins[J],2009.
APA	刘光明,王玉松,黄园园,曹敏杰,张凌晶,&苏文金.(2009).辐照处理对蟹类过敏原(原肌球蛋白)性质的影响..
MLA	刘光明,et al."辐照处理对蟹类过敏原(原肌球蛋白)性质的影响".(2009).

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