Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus

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	Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus
	Lu, Zhonglei ; Kong, Xiangxiang ; Lu, Zhaoming ; Xiao, Meixiang ; Chen, Meiyuan ; Zhu, Liang ; Shen, Yuemao ; Hu, Xiangyang ; Song, Siyang ; Shen YM(沈月毛) ; Song SY(宋思扬)
刊名	http://dx.doi.org/10.1371/journal.pone.0091298
	2014-03-10
关键词	PRECURSOR P-AMINOBENZOATE SALICYLIC-ACID TEMPERATURE STRESS OXIDATIVE STRESS GENE-EXPRESSION PROTECTIVE ROLE ABIOTIC STRESS GLUCOSE ESTER ABSCISIC-ACID SALT STRESS
英文摘要	100 Talents Program of the Chinese Academy of Sciences; National Science Foundation of China [30571295, 30871704, 30971452, 31170256]; Major Science and Technology Program [10201101003-TS-03]; Most mushrooms are thermo-sensitive to temperatures over 23 degrees C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermosensitive. Here, we compared their responses at proteomic level to heat treatment at 33 degrees C. We identified 73 proteins that are differentially expressed between 02 and 8213 or induced upon heat stress in strain 02 itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in 02 than 8213; and they can be further upregulated in response to heat stress in 02, but not in 8213. One protein is encoded by the para-aminobenzoic acid (PABA) synthase gene Pabs, which has been shown to scavenge the reactive oxygen species in vitro. Pabs mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in 02, indicating transcriptional level upregulation of Pabs upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of 02, while exogenous PABA or transgenic overexpression of 02 derived PABA synthase enhanced thermotolerance of 8213. Furthermore, compared to 8213, 02 accumulated less H2O2 but more defense-related proteins (e. g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H2O2 and elevating defense-related proteins.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90818]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Lu, Zhonglei,Kong, Xiangxiang,Lu, Zhaoming,et al. Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus[J]. http://dx.doi.org/10.1371/journal.pone.0091298,2014.
APA	Lu, Zhonglei.,Kong, Xiangxiang.,Lu, Zhaoming.,Xiao, Meixiang.,Chen, Meiyuan.,...&宋思扬.(2014).Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus.http://dx.doi.org/10.1371/journal.pone.0091298.
MLA	Lu, Zhonglei,et al."Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus".http://dx.doi.org/10.1371/journal.pone.0091298 (2014).