PCR screening reveals considerable unexploited biosynthetic potential of ansamycins and a mysterious family of AHBA-containing natural products in actinomycetes

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	PCR screening reveals considerable unexploited biosynthetic potential of ansamycins and a mysterious family of AHBA-containing natural products in actinomycetes
	Wang, H. -X. ; Chen, Y. -Y. ; Ge, L. ; Fang, T. -T. ; Meng, J. ; Liu, Z. ; Fang, X. -Y. ; Ni, S. ; Lin, C. ; Wu, Y. -Y. ; Wang, M. -L. ; Shi, N. -N. ; He, H. -G. ; Hong, K. ; Shen, Y. -M. ; Shen YM(沈月毛)
刊名	http://dx.doi.org/10.1111/jam.12217
	2013
关键词	ANTIBIOTIC MITOMYCIN-C 3-AMINO-5-HYDROXYBENZOIC ACID GENE-CLUSTER STREPTOMYCES-HYGROSCOPICUS MOLECULAR CHARACTERIZATION GELDANAMYCIN SYNTHASE MICROMONOSPORA IDENTIFICATION ANSAMITOCIN
英文摘要	973 Programs [2010CB833802, 2012CB721005]; NSFC [31100079]; Independent Innovation Foundation of Shandong University [2010TS072]; Aims Ansamycins are a family of macrolactams that are synthesized by type I polyketide synthase (PKS) using 3-amino-5-hydroxybenzoic acid (AHBA) as the starter unit. Most members of the family have strong antimicrobial, antifungal, anticancer and/or antiviral activities. We aimed to discover new ansamycins and/or other AHBA-containing natural products from actinobacteria. Methods and Results Through PCR screening of AHBA synthase gene, we identified 26 AHBA synthase gene-positive strains from 206 plant-associated actinomycetes (five positives) and 688 marine-derived actinomycetes (21 positives), representing a positive ratio of 2 center dot 4-3 center dot 1%. Twenty-five ansamycins, including eight new compounds, were isolated from six AHBA synthase gene-positive strains through TLC-guided fractionations followed by repeated column chromatography. To gain information about those potential ansamycin gene clusters whose products were unknown, seven strains with phylogenetically divergent AHBA synthase genes were subjected to fosmid library construction. Of the seven gene clusters we obtained, three show characteristics for typical ansamycin gene clusters, and other four, from Micromonospora spp., appear to lack the amide synthase gene, which is unusual for ansamycin biosynthesis. The gene composition of these four gene clusters suggests that they are involved in the biosynthesis of a new family of hybrid PK-NRP compounds containing AHBA substructure. Conclusions PCR screening of AHBA synthase is an efficient approach to discover novel ansamycins and other AHBA-containing natural products. Significance and Impact of the Study This work demonstrates that the AHBA-based screening method is a useful approach for discovering novel ansamycins and other AHBA-containing natural products from new microbial resources.
语种	英语
出版者	WILEY-BLACKWELL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90706]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Wang, H. -X.,Chen, Y. -Y.,Ge, L.,et al. PCR screening reveals considerable unexploited biosynthetic potential of ansamycins and a mysterious family of AHBA-containing natural products in actinomycetes[J]. http://dx.doi.org/10.1111/jam.12217,2013.
APA	Wang, H. -X..,Chen, Y. -Y..,Ge, L..,Fang, T. -T..,Meng, J..,...&沈月毛.(2013).PCR screening reveals considerable unexploited biosynthetic potential of ansamycins and a mysterious family of AHBA-containing natural products in actinomycetes.http://dx.doi.org/10.1111/jam.12217.
MLA	Wang, H. -X.,et al."PCR screening reveals considerable unexploited biosynthetic potential of ansamycins and a mysterious family of AHBA-containing natural products in actinomycetes".http://dx.doi.org/10.1111/jam.12217 (2013).