DNA Topoisomerase II Is Dispensable for Oocyte Meiotic Resumption but Is Essential for Meiotic Chromosome Condensation and Separation in Mice

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	DNA Topoisomerase II Is Dispensable for Oocyte Meiotic Resumption but Is Essential for Meiotic Chromosome Condensation and Separation in Mice
	Li, Xiao-Meng ; Yu, Chao ; Wang, Zhong-Wei ; Zhang, Yin-Li ; Liu, Xiao-Man ; Zhou, Dawang ; Sun, Qing-Yuan ; Fan, Heng-Yu ; Zhou DW(周大旺)
刊名	http://dx.doi.org/10.1095/biolreprod.113.110692
	2013
关键词	EMBRYONIC STEM-CELLS DECATENATION CHECKPOINT MAMMALIAN OOCYTES CYCLE CHECKPOINT CHROMATIN DAMAGE ALPHA MICROTUBULES MATURATION SURVIVIN
英文摘要	National Basic Research Program of China [2011CB944504, 2012CB944403]; National Natural Science Foundation of China [81172473]; Natural Science Foundation of Zhejiang Province [R2100145]; Basic Scientific Research Funding of Zhejiang University [2011QN81001]; Open Research Fund of State Key Laboratory of Cellular Stress Biology, Xiamen University [SKLCSB2013KF001]; During mitosis, DNA topoisomerase II (TOP2) is required for sister chromatid separation. When TOP2 activity is inhibited, a decatenation checkpoint is activated by entangled chromatin. However, the functions of TOP2 in oocyte meiosis, particularly for homologous chromosome segregation during meiosis I, have not been investigated. In addition, it remains unknown if TOP2 inhibition activates a decatenation checkpoint at the G2/M transition in oocytes. In this study, we used mouse oocytes and specific inhibitors of TOP2 (ICRF-193 and etoposide) to investigate the role of TOP2 in meiosis. Our results indicated that an effective decatenation checkpoint did not exist in fully grown oocytes, as oocytes underwent the G2/M transition and reinitiated meiosis even when TOP2 activity was inhibited. However, oocytes treated with ICRF-193 had severe defects in chromosome condensation and homologous chromosome separation. Furthermore, condensed chromosomes failed to maintain their normal configurations in matured oocytes that were treated with ICRF-193. However, sister chromatid separation and subsequent chromosome decondensation during the exit from meiosis were not blocked by TOP2 inhibitors. These results indicated that TOP2 had a specific, crucial function in meiosis I. Thus, we identified important functions of TOP2 during oocyte maturation and provided novel insights into the decatenation checkpoint during meiosis.
语种	英语
出版者	SOC STUDY REPRODUCTION
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90659]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Li, Xiao-Meng,Yu, Chao,Wang, Zhong-Wei,et al. DNA Topoisomerase II Is Dispensable for Oocyte Meiotic Resumption but Is Essential for Meiotic Chromosome Condensation and Separation in Mice[J]. http://dx.doi.org/10.1095/biolreprod.113.110692,2013.
APA	Li, Xiao-Meng.,Yu, Chao.,Wang, Zhong-Wei.,Zhang, Yin-Li.,Liu, Xiao-Man.,...&周大旺.(2013).DNA Topoisomerase II Is Dispensable for Oocyte Meiotic Resumption but Is Essential for Meiotic Chromosome Condensation and Separation in Mice.http://dx.doi.org/10.1095/biolreprod.113.110692.
MLA	Li, Xiao-Meng,et al."DNA Topoisomerase II Is Dispensable for Oocyte Meiotic Resumption but Is Essential for Meiotic Chromosome Condensation and Separation in Mice".http://dx.doi.org/10.1095/biolreprod.113.110692 (2013).