HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries

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	HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries
	Zhou, Sisi ; Fu, Yonggui ; Li, Jie ; He, Lingyu ; Cai, Xingsheng ; Yan, Qingyu ; Rao, Xingqiang ; Huang, Shengfeng ; Li, Guang ; Wang, Yiquan ; Xu, Anlong ; Wang YQ(王义权)
刊名	http://dx.doi.org/10.1371/journal.pone.0052257
	2012-12-20
关键词	STRUCTURAL VARIATION BAC LIBRARY CONSTRUCTION GENERATION EVOLUTION DNA
英文摘要	National Natural Science Foundation of China [30730089, 30801012, 31171193]; National Basic Research Program of China (973 Program) [2011CB946101, 2007CB815800]; Fundamental Research Funds for the Central Universities; State High-Tech Development Project (863 Project) [2008AA092601]; Second generation sequencing has been widely used to sequence whole genomes. Though various paired-end sequencing methods have been developed to construct the long scaffold from contigs derived from shotgun sequencing, the classical paired-end sequencing of the Bacteria Artificial Chromosome (BAC) or fosmid libraries by the Sanger method still plays an important role in genome assembly. However, sequencing libraries with the Sanger method is expensive and time-consuming. Here we report a new strategy to sequence the paired-ends of genomic libraries with parallel pyrosequencing, using a Chinese amphioxus (Branchiostoma belcheri) BAC library as an example. In total, approximately 12,670 non-redundant paired-end sequences were generated. Mapping them to the primary scaffolds of Chinese amphioxus, we obtained 413 ultra-scaffolds from 1,182 primary scaffolds, and the N50 scaffold length was increased approximately 55 kb, which is about a 10% improvement. We provide a universal and cost-effective method for sequencing the ultra-long pairedends of genomic libraries. This method can be very easily implemented in other second generation sequencing platforms.
语种	英语
出版者	PLOS ONE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90578]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhou, Sisi,Fu, Yonggui,Li, Jie,et al. HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries[J]. http://dx.doi.org/10.1371/journal.pone.0052257,2012.
APA	Zhou, Sisi.,Fu, Yonggui.,Li, Jie.,He, Lingyu.,Cai, Xingsheng.,...&王义权.(2012).HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries.http://dx.doi.org/10.1371/journal.pone.0052257.
MLA	Zhou, Sisi,et al."HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries".http://dx.doi.org/10.1371/journal.pone.0052257 (2012).