Purification and characterization of beta-1,4-glucosidase from Aspergillus glaucus

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	Purification and characterization of beta-1,4-glucosidase from Aspergillus glaucus
	Ma, Su-Juan ; Leng, Bo ; Xu, Xin-Qi ; Zhu, Xiang-Zhi ; Shi, Yan ; Tao, Yi-Ming ; Chen, Shao-Xuan ; Long, Min-Nan ; Chen, Qing-Xi ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.5897/AJB11.2144
	2011-12-26
关键词	THERMOSTABLE BETA-GLUCOSIDASE THERMOPHILIC FUNGUS BIOCHEMICAL-CHARACTERIZATION PENICILLIUM-OCCITANIS CELLULOSE ENDOGLUCANASE EXPRESSION SUBSTRATE PROTEINS CLONING
英文摘要	National Key Basic Research Program (NKBRP) [2010CB732201]; Natural Science Foundation of Fujian Province [2009J01196]; National Foundation for fostering talents of basic science [J1030626]; A beta-1,4-glucosidase (BG) was purified from fermentation liquor of Aspergillus glaucus by the following procedures: Ammonium sulfate precipitation, gel filtration on Sephadex G-100, and hydrophobic chromatography on Phenyl Sepharose Fast Flow. The specific activity on salicin was determined as 1747.4 U/mg. The molecular weight of this enzyme was determined as 23.5 and 92.5 kDa with SDS-PAGE and gel filtration on Sephadex G-100, respectively indicating that BG is a tetramer. The enzyme was stable at the pH ranging from 2.2 to 7.5, and its maximum activity was obtained at pH 3.6. The enzymatic activity gradually increased in the range from 40 to 60 degrees C, but a sharp decrease occurred at 65 degrees C. Enzymatic kinetics indicated that Michaelis-Menten contant (K(m)) of the hydrolysis for salicin by BG was 2.58 mmol/L (pH 3.6, 60 degrees C). Na(+), K(+), Ca(2+), Mg(2+), Ba(2+), NO(3)(-) and SO(4)(2-) had no effects on BG activity. The enzyme activity was activated by Mn(2+) and Fe(2+), while it was strongly inhibited by Cu(2+), Pb(2+), Cd(2+), SDS and EDTA, and slightly inhibited by Zn(2+).
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65657]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ma, Su-Juan,Leng, Bo,Xu, Xin-Qi,et al. Purification and characterization of beta-1,4-glucosidase from Aspergillus glaucus[J]. http://dx.doi.org/10.5897/AJB11.2144,2011.
APA	Ma, Su-Juan.,Leng, Bo.,Xu, Xin-Qi.,Zhu, Xiang-Zhi.,Shi, Yan.,...&陈清西.(2011).Purification and characterization of beta-1,4-glucosidase from Aspergillus glaucus.http://dx.doi.org/10.5897/AJB11.2144.
MLA	Ma, Su-Juan,et al."Purification and characterization of beta-1,4-glucosidase from Aspergillus glaucus".http://dx.doi.org/10.5897/AJB11.2144 (2011).