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	Denaturation Kinetics of N-Acetyl/beta-D-glucosaminidase from Scylla serrata in Urea Solution
	Lin Jian-cheng ; Li Hua-liang ; Chen Liang-hua ; Yang Xue-min ; Wang Qin ; Chen Qing-xi ; Chen QX(陈清西)
	2011-09
关键词	PRAWN PENAEUS-VANNAMEI BETA-D-GLUCOSAMINIDASE GUANIDINE-HYDROCHLORIDE INACTIVATION KINETICS CONFORMATIONAL-CHANGE INHIBITORY KINETICS AMINOACYLASE CHLORIDE
英文摘要	National Natural Science Foundation of China [40576066]; Science and Technology Foundation of Xiamen, China [3502Z20081143]; N-Acetyl-beta-D-glucosaminidase(NAGase, EC 3.2.1.52), which catalyzes the cleavage of N-acetylglucosamine polymers, plays important roles in the molting, digestion of chitinous foods in green crab. In the study, the efforts of urea on the activity of NAGase purified from the viscera of green crab(Scylla serrata) have been studied. The results show that appropriate concentrations of urea can lead to reversible inactivation of the enzyme, and the value of the inhibitor concentration leading to 50% of enzyme activity lost(IC(50)) is estimated to be 0.63 mol/L. The inactivation kinetics has been studied via the kinetic method of the substrate reaction. The rate constants of inactivation have been determined. The value of k(+0) is larger than that of k'(+0), indicating the free enzyme molecule is more fragile than the enzyme-substrate complex in urea solution. It is suggested that the presence of the substrate offers the marked protection of this enzyme against inactivation by urea.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65638]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Lin Jian-cheng,Li Hua-liang,Chen Liang-hua,et al. Denaturation Kinetics of N-Acetyl/beta-D-glucosaminidase from Scylla serrata in Urea Solution[J],2011.
APA	Lin Jian-cheng.,Li Hua-liang.,Chen Liang-hua.,Yang Xue-min.,Wang Qin.,...&陈清西.(2011).Denaturation Kinetics of N-Acetyl/beta-D-glucosaminidase from Scylla serrata in Urea Solution..
MLA	Lin Jian-cheng,et al."Denaturation Kinetics of N-Acetyl/beta-D-glucosaminidase from Scylla serrata in Urea Solution".(2011).

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