Roles of phospholipase D in phorbol myristate acetate-stimulated neutrophil respiratory burst

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	Roles of phospholipase D in phorbol myristate acetate-stimulated neutrophil respiratory burst
	Tianhui Hu ; Hu TH(胡天惠) ; Zhaoxiao Liu ; Xun Shen
刊名	http://dx.doi.org/10.1111/j.1582-4934.2010.01035.x
	2011-03
关键词	PROTEIN-KINASE-C PHOSPHATIDIC-ACID PRODUCTION D ACTIVATION LEUCYL-PHENYLALANINE SIGNAL-TRANSDUCTION CELLS CALCIUM MECHANISMS GENERATION INHIBITOR
英文摘要	National Natural Science Foundation of China [39970197, 30971524]; Xiamen University; Ministry of Science and Technology [2009CB522200, 2006AA02A303]; The phorbol myristate acetate (PMA) stimulated nutrophil respiratory burst has been considered to simply involve the activation of protein kinase C (PKC). However, the PLD activity was also increased by 10-fold in human neutrophils stimulated with 100 nM PMA. Unexpectedly, U73122, an inhibitor of phospholipase C, was found to significantly inhibit PMA-stimulated respiratory burst in human neutrophils. U73122 at the concentrations, which were sufficient to inhibit the respiratory burst completely, caused partial inhibition of the PLD activity but no inhibition on PKC translocation and activation, suggesting that PLD activity is also required in PMA-stimulated respiratory burst. Using 1-butanol, a PLD substrate, to block phosphatidic acid (PA) generation, the PMA-stimulated neutrophil respiratory burst was also partially inhibited, further indicating that PLD activation, possibly its hydrolytic product PA and diacylglycerol (DAG), is involved in PMA-stimulated respiratory burst. Since GF109203X, an inhibitor of PKC that could completely inhibit the respiratory burst in PMA-stimulated neutrophils, also caused certain suppression of PLD activation, it may suggest that PLD activation in PMA-stimulated neutrophils might be, to some extent, PKC dependent. To further study whether PLD contributes to the PMA stimulated respiratory burst through itself or its hydrolytic product, 1,2-dioctanoyl-sn-glycerol, an analogue of DAG , was used to prime cells at low concentration, and it reversed the inhibition of PMA-stimulated respiratory burst by U73122. The results indicate that U73122 may act as an inhibitor of PLD, and PLD activation is required in PMA-stimulated respiratory burst.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65448]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Tianhui Hu,Hu TH,Zhaoxiao Liu,et al. Roles of phospholipase D in phorbol myristate acetate-stimulated neutrophil respiratory burst[J]. http://dx.doi.org/10.1111/j.1582-4934.2010.01035.x,2011.
APA	Tianhui Hu,胡天惠,Zhaoxiao Liu,&Xun Shen.(2011).Roles of phospholipase D in phorbol myristate acetate-stimulated neutrophil respiratory burst.http://dx.doi.org/10.1111/j.1582-4934.2010.01035.x.
MLA	Tianhui Hu,et al."Roles of phospholipase D in phorbol myristate acetate-stimulated neutrophil respiratory burst".http://dx.doi.org/10.1111/j.1582-4934.2010.01035.x (2011).