Using population dynamics analysis by DGGE to design the bacterial consortium isolated from mangrove sediments for biodegradation of PAHs

CORC > 厦门大学 > 生命科学－已发表论文

	Using population dynamics analysis by DGGE to design the bacterial consortium isolated from mangrove sediments for biodegradation of PAHs
	Liu, H. J. ; Yang, C. Y. ; Tian, Y. ; Lin, G. H. ; Zheng, T. L. ; Lin GH(林光辉)
刊名	http://dx.doi.org/10.1016/j.ibiod.2010.11.010
	2011-03
关键词	POLYCYCLIC AROMATIC-HYDROCARBONS DEGRADING BACTERIA MICROBIAL COMMUNITY HONG-KONG CONTAMINATED SOIL SPHINGOMONAS SP BIOREMEDIATION DEGRADATION OIL PHENANTHRENE
英文摘要	National Natural Science Foundation of China [40976069, 40930847, 30710103908, 30930017, 30940002]; Science and Technology Foundation of Fujian Province [2008Y0061]; There are many PAH-degrading bacteria in mangrove sediments and in order to explore their degradation potential, surface sediment samples were collected from a mangrove area in Fugong, Longhai, Fujian Province of China. A total of 53 strains of PAH-degrading bacteria were isolated from the mangrove sediments, consisting of 14 strains of phenanthrene (Phe), 13 strains of pyrene (Pyr), 13 strains of benzo [a]pyrene (Bap) and 13 strains of mixed PAH (Phe + Pyr + Bap)-degrading bacteria. All of the individual colonies were identified by 165 rDNA sequencing. Based on the information of bacterial PCR-DGGE profiles obtained during enrichment batch culture, Phe, Pyr, Bap and mixed PAH-degrading consortia consisted of F1, F2, F3, F4 and F15 strains, B1, 83, 86, 87 and B13 strains, P1, P2, P3, P5 and P7 strains, M1, M2, M4, M12 and M13 strains, respectively. In addition, the degradation ability of these consortia was also determined. The results showed that both Phe and mixed PAH-degrading consortia had the highest ability to degrade the Phe in a liquid medium, with more than 91% being degraded in 3 days. But the biodegradation percentages of Pyr by Pyr-degrading consortium and Bap by Bap-degrading consortium were relatively lower than that of the Phe-degrading consortium. These results suggested that a higher degradation of PAHs depended on both the bacterial consortium present and the type of PAH compound. Moreover, using the bacterial community structure analysis method, where the consortia consist of different PAH-degrading bacteria, the information from the PCR-DGGE profiles could be used in the bioremediation of PAHs in the future. (C) 2010 Elsevier Ltd. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65277]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liu, H. J.,Yang, C. Y.,Tian, Y.,et al. Using population dynamics analysis by DGGE to design the bacterial consortium isolated from mangrove sediments for biodegradation of PAHs[J]. http://dx.doi.org/10.1016/j.ibiod.2010.11.010,2011.
APA	Liu, H. J.,Yang, C. Y.,Tian, Y.,Lin, G. H.,Zheng, T. L.,&林光辉.(2011).Using population dynamics analysis by DGGE to design the bacterial consortium isolated from mangrove sediments for biodegradation of PAHs.http://dx.doi.org/10.1016/j.ibiod.2010.11.010.
MLA	Liu, H. J.,et al."Using population dynamics analysis by DGGE to design the bacterial consortium isolated from mangrove sediments for biodegradation of PAHs".http://dx.doi.org/10.1016/j.ibiod.2010.11.010 (2011).