A three-dimensional flow control concept for single-cell experiments on a microchip 1 Cell selection, cell retention, cell culture, cell balancing, and cell scanning

CORC > 厦门大学 > 生命科学－已发表论文

	A three-dimensional flow control concept for single-cell experiments on a microchip 1 Cell selection, cell retention, cell culture, cell balancing, and cell scanning
	Peng, X. Y. ; Li, P. C. H. ; Peng XY(彭兴跃)
刊名	http://dx.doi.org/10.1021/ac049384s
	2004-09-15
关键词	MICROFLUIDIC DEVICE SILICON CHIP LYSIS MANIPULATION PARTICLES TRANSPORT CHANNELS SYSTEMS SORTER
英文摘要	An ideal microchip for single-cell experiments should be able to allow us to culture cells, to select any desired single cell from a group, to retain the cell for convenient cellular signal detection, and to deliver any buffer or reagent directly to the cell at any time during continual detection and observation. Most importantly, any negative impact on the live cell should be minimized. To accomplish all these functions, we developed a three-dimensional liquid flow control concept and employed special liquid flow fields to manipulate and retain a single yeast cell freely in the chip. A zero-speed point was controlled to retain the cell for three-dimensional cell balancing and cell scanning. A dispersive flow delivered reagents at a high speed to very near the cell and provided them to the cell at a low speed. No force stronger than its gravitational force was exerted on the cell, which could be balanced on different positions on an arc-sloping wall, thus minimizing any negative impact on the cell due to strong liquid flows. Specifically, we demonstrate on-chip single-cell culture, cell wall removal, and reagent delivery. Subsequently, single-cell fluorescence detection was performed, and noise filtering and background correction were applied for data processing.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65119]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Peng, X. Y.,Li, P. C. H.,Peng XY. A three-dimensional flow control concept for single-cell experiments on a microchip 1 Cell selection, cell retention, cell culture, cell balancing, and cell scanning[J]. http://dx.doi.org/10.1021/ac049384s,2004.
APA	Peng, X. Y.,Li, P. C. H.,&彭兴跃.(2004).A three-dimensional flow control concept for single-cell experiments on a microchip 1 Cell selection, cell retention, cell culture, cell balancing, and cell scanning.http://dx.doi.org/10.1021/ac049384s.
MLA	Peng, X. Y.,et al."A three-dimensional flow control concept for single-cell experiments on a microchip 1 Cell selection, cell retention, cell culture, cell balancing, and cell scanning".http://dx.doi.org/10.1021/ac049384s (2004).