| 深圳市耐多药结核分枝杆菌流行株耐药基因序列分析; Analyze the epidemical multidrug-resistant Mycobacterium tuberculosis from Shenzhen by DNA sequencing | |
| 崔运勇(深圳市慢性病防治中心) ; 王峰(深圳市慢性病防治中心) ; 刘小立(深圳市慢性病防治中心) ; 杨慧(深圳市慢性病防治中心) ; 桂静(深圳市慢性病防治中心) ; 胡思玉 | |
| 2011-05 | |
| 关键词 | 分枝杆菌 结核 抗药性 多种 细菌 序列分析 DNA 突变 深圳市 Mycobacterium tuberculosis Drug resistance mutiple bacterial sequence analysis mutation Shenzhen city |
| 英文摘要 | 通讯作者: 杨慧( yh2009cn@y ahoo.com.cn); [中文文摘]目的了解深圳市耐多药结核分枝杆菌(multidrug-resistant Mycobacterium tuberculosis,MDR-TB)的分子流行病学特征。方法参照WHO/I UATLD标准,使用L-J药敏培养基,采用1%比例法药敏试验筛选出敏感株和异烟肼(isoniazid,INH)、利福平(rifampicin,RFP)双耐药临床分离株,通过DNA测序检测深圳地区153株敏感株与132株MDR-TB的INH耐药基因katG、inhA、oxyR-ahpC基因间区域及RFP耐药基因rpoB碱基排列顺序,运用DNASTAR和blastn进行序列分析。结果 153株敏感株突变率为27.5%(42/153)。132株MDR-TB突变率为98.5%(130/132),其中katG基因突变率为73.5%(97/132),68.9%(91/132)为katG315位突变;inhA基因突变率为18.2%(24/132),11.4%(15/132)为启动子区域突变,未发现inhA94特异突变株;ahpC基因突变率为16.7%(22/132),10.6%(14/132)为启动子区域突变;rpoB基因81 bp核心区域突变率为93.2%(123/132)。结论 katG315、inhA启动子区域、ahpC启动子区域以及rpoB 81 bp核心区域突变为深圳地区耐多药结核分枝杆菌主要突变类型,与其他国家和地区差异无统计学意义;但深圳地区未见inhA94突变株。[英文文摘]Objective To investigate the epidemiologic characteristic of mult idrug-resistant (MDR) Mycobacterium tuberculosis (MTB) in Shenzhen. Methods According to the standard of WHO, International Union Against Tuberculosis and Lung Disease (IU ATLD) ,drug-susceptive and MDR MTB strains were collected by drug suscept ibility test (DST) of 1% proportion method.kat G, inh A, intergenic region of oxy R-ahp C and 81bp core region of rpo B genes in 153 drug-sus-ceptive strains and 132 MDR strains were analyzed by DNA sequencing. Results 27.5%(42/153) of drug-susceptive strains and 98.5% (130/132) of MDR strains showed gene mutat ions. Of 132 MDR strains, 73.5%( 97/132) had ka t G mutat ions, and 68.9% (91/132) of kat G mutations were at codon 315; 18.2% (24/132) had in h A mutations, and none specific mutations were found at codon 94 of in h A; 11.4% (15/132) had mutations at inh A-promoter region; 16.7% (22/132) had ahp C mutat ions, 10.6% (14/132) had mutations at interg enic region of oxyR-ah p C; 93.2%( 123/132) had mutations at the 81bp core region of rpoB. Conclusion The codon 315 of kat G, promoter region of inh A, ahp C and the 81bp core region of rpoB play predominant roles in MDR-T B in Shenzhen.; 艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2008ZX10003-004) |
| 语种 | 中文 |
| 出版者 | 《中国防痨杂志》编辑部 |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/18842]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 崔运勇(深圳市慢性病防治中心),王峰(深圳市慢性病防治中心),刘小立(深圳市慢性病防治中心),等. 深圳市耐多药结核分枝杆菌流行株耐药基因序列分析, Analyze the epidemical multidrug-resistant Mycobacterium tuberculosis from Shenzhen by DNA sequencing[J],2011. |
| APA | 崔运勇,王峰,刘小立,杨慧,桂静,&胡思玉.(2011).深圳市耐多药结核分枝杆菌流行株耐药基因序列分析.. |
| MLA | 崔运勇,et al."深圳市耐多药结核分枝杆菌流行株耐药基因序列分析".(2011). |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论