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	Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells
	Kinjo, Tomoya ; Ye, Jun ; Ye J(叶军) ; Yan, Hanxu ; Hamasaki, Takeki ; Nakanishi, Hidekazu ; Toh, Kazuko ; Nakamichi, Noboru ; Kabayama, Shigeru ; Teruya, Kiichiro ; Shirahata, Sanetaka
	2012-05
关键词	Cell invasion Electrochemically reduced water Matrix metalloproteinase-2 HT1080 cells Hydrogen p38 mitogen-activated protein kinase Platinum nanoparticles
英文摘要	It has been demonstrated that hydrogen peroxide (H2O2) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines. Electrochemically reduced water (ERW), produced near the cathode during electrolysis, and scavenges intracellular H2O2 in human fibrosarcoma HT1080 cells. RT-PCR and zymography analyses revealed that when HT1080 cells were treated with ERW, the gene expression of MMP-2 and membrane type 1 MMP and activation of MMP-2 was repressed, resulting in decreased invasion of the cells into matrigel. ERW also inhibited H2O2-induced MMP-2 upregulation. To investigate signal transduction involved in MMP-2 downregulation, mitogen-activated protein kinase (MAPK)-specific inhibitors, SB203580 (p38 MAPK inhibitor), PD98059 (MAPK/extracellular regulated kinase kinase 1 inhibitor) and c-Jun NH2-terminal kinase inhibitor II, were used to block the MAPK signal cascade. MMP-2 gene expression was only inhibited by SB203580 treatment, suggesting a pivotal role of p38 MAPK in regulation of MMP-2 gene expression. Western blot analysis showed that ERW downregulated the phosphorylation of p38 both in H2O2-treated and untreated HT1080 cells. These results indicate that the inhibitory effect of ERW on tumor invasion is due to, at least in part, its antioxidative effect.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1007/s10616-012-9469-7]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Kinjo, Tomoya,Ye, Jun,Ye J,et al. Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells[J],2012.
APA	Kinjo, Tomoya.,Ye, Jun.,叶军.,Yan, Hanxu.,Hamasaki, Takeki.,...&Shirahata, Sanetaka.(2012).Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells..
MLA	Kinjo, Tomoya,et al."Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells".(2012).

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