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	New primers for sex identification in the Chinese Egret and other ardeid species
	Wang, Zeng ; Zhou, Xiaoping ; Zhou XP(周晓平) ; Lin, Qingxian ; Lin QX(林清贤) ; Fang, Wenzhen ; Fang WZ(方文珍) ; Chen, Xiaolin ; Chen XL(陈小麟)
	2010-05-17
关键词	ardeid CHD gene primer sex identification
英文摘要	Using the universal P2/P8 primers, we were able to obtain the gene segments of chromo-helicase-DNA binding protein (CHD)-Z and CHD-W from ten species of ardeid birds including Chinese egret (Egretta eulophotes), little egret (E. garzetta), eastern reef egret (E. sacra), great egret (Ardea alba), grey heron (A. cinerea), Chinese pond-heron (Ardeola bacchus), cattle egret (Bubulcus ibis), black-crowned night-heron (Nycticorax nycticorax), cinnamon bittern (Ixobrychus cinnamomeus) and yellow bittern (I. sinensis). Based on conserved regions inside the P2/P8-derived sequences, we designed new PCR primers for sex identification in these ardeid species. Using agarose gel electrophoresis, the PCR products showed two bands for females (140 bp derived from CHD-W and the other 250 bp from CHD-ZW), whereas the males showed only the 250 bp band. The results indicated that our new primers could be used for accurate and convenient sex identification in ardeid species.; National Natural Science Foundation of China[30970380, 40876077]; Fujian Natural Science Foundation of China[2008S0007, 2009J01195]
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1186/gb-2011-12-4-r33]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Wang, Zeng,Zhou, Xiaoping,Zhou XP,et al. New primers for sex identification in the Chinese Egret and other ardeid species[J],2010.
APA	Wang, Zeng.,Zhou, Xiaoping.,周晓平.,Lin, Qingxian.,林清贤.,...&陈小麟.(2010).New primers for sex identification in the Chinese Egret and other ardeid species..
MLA	Wang, Zeng,et al."New primers for sex identification in the Chinese Egret and other ardeid species".(2010).

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