QQ客服 官方微博 反馈留言

	Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution
	Tao, Yi-Ming ; Xu, Xin-Qi ; Ma, Su-Juan ; Liang, Ge ; Wu, Xiao-Bing ; Long, Min-Nan ; Long MN(龙敏南) ; Chen, Qing-Xi ; Chen QX(陈清西)
	2011-09-15
关键词	cellulase hydrolysis endoglucanase denaturation urea Aspergillus
英文摘要	In order to optimize the cellulase (from Aspergillus glaucus) hydrolysis of pretreated rice straw, the effects of varying enzyme concentration, temperature, and pH were studied. The best experimental conditions found to degrade the pretreated rice straws were 24 h of incubation at 55 degrees C and pH 5.0, with an enzyme concentration of 48 mg/L. Urea is one of the important nitrogen sources used in fungi culture, but it is also a denaturant. The model of denaturation of endoglucanase (EG) in urea solutions was established. The denaturation was a slow, reversible reaction. Determination of microscopic rate constants showed k(+0) > K'(+0) indicating that EG was protected by the substrate to a certain extent during denaturation. Comparison with the results from fluorescence emission spectroscopy revealed that the inactivation of EG occurred before the marked conformational changes could be detected.; National Foundation for Fostering Talents of Basic Science[J1030626]; National Key Basic Research Program (NKBRP)[2010CB732201]; Natural Science Foundation of Fujian Province[2009J01196]
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1021/jf203712n]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Tao, Yi-Ming,Xu, Xin-Qi,Ma, Su-Juan,et al. Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution[J],2011.
APA	Tao, Yi-Ming.,Xu, Xin-Qi.,Ma, Su-Juan.,Liang, Ge.,Wu, Xiao-Bing.,...&陈清西.(2011).Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution..
MLA	Tao, Yi-Ming,et al."Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution".(2011).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。