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	Evaluation of drug effects on Toxoplasma gondii nuclear and plastid DNA replication using real-time PCR
	Zhao, Qing ; Hong, Lingxian ; Hong LX(洪凌仙) ; Zhou, Kefu ; Zhou KF(周克夫) ; Lin, Yuguang ; Zhang, Ming
	2010
英文摘要	Toxoplasma gondii Nicolle and Manceaux, 1908 is a unicellular protozoan that can infect a broad spectrum of organisms including humans. In addition to a nuclear genome, it also carries a circular DNA within a plastid-like organelle (apicoplast) and a linear genome within its mitochondria. The plastid organelle has been shown to be the target of various anti-parasitic drugs or antibiotics. To evaluate the effects of agents on the DNA replication of T. gondii, we tested six drugs (ciprofloxacin, acetylspiramycin, clindamycin, azithromycin, artemether, and sulfadiazine) on the parasite cultured in Hela cells. After drug treatment for 48 h, the parasite growth and DNA replication were evaluated and quantitated using TaqMan real-time quantitative PCR with oligonucleotide primers synthesized based on a gene from the apicoplast genome (ycf24, Genbank accession no. U87145) and a gene from the nuclear genome (uprt, Genbank accession no. U10246). Our results showed that ciprofloxacin was the most effective in inhibiting the replication of the plastid DNA after 48 h drug treatment, with a reduction of 22% in the copy number of the plastid DNA. Artemether was the most effective drug in suppressing the proliferation of tachyzoites. This study also demonstrates that real-time quantitative PCR is a simple and useful technique for monitoring parasite growth and DNA replication.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1007/s00436-010-1792-3]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhao, Qing,Hong, Lingxian,Hong LX,et al. Evaluation of drug effects on Toxoplasma gondii nuclear and plastid DNA replication using real-time PCR[J],2010.
APA	Zhao, Qing.,Hong, Lingxian.,洪凌仙.,Zhou, Kefu.,周克夫.,...&Zhang, Ming.(2010).Evaluation of drug effects on Toxoplasma gondii nuclear and plastid DNA replication using real-time PCR..
MLA	Zhao, Qing,et al."Evaluation of drug effects on Toxoplasma gondii nuclear and plastid DNA replication using real-time PCR".(2010).

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